JPK NanoWizard V BioScience AFM

NanoWizard® V BioScience

The JPK NanoWizard® V combines high spatio-temporal resolution with a large scan area, flexible experiment design, and out-standing integration with advanced optical microscope systems. The automated setup, alignment, and re-adjustment of system parameters opens new possibilities for long-term, self-regulating experiment series.

Automation

Perfecting performance, increasing productivity

Automated setup, workflow, and calibration opens new possibilities for long-term, self-regulating experiment series and complex experimental routines.

NanoMechanics

Quantitative Imaging

Nanomechanical characterization of single molecules, cells, tissues, and highly delicate samples thanks to advanced force control.

Fast scanning

400 lines/sec

Study dynamic biological processes in real time via adaptive, intelligence-based scanning routines, fast force mapping and molecular recognition.

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Discover the 5th Generation BioAFM

NanoWizard V is expected to significantly advance our understanding of dynamic cellular processes and molecular mechanisms. Its PeakForce-QI mode enables fast and flexible quantitative nanomechanical measurements—significantly extending the capabilities of AFM—while its automated, remote-control, and fast-scanning capabilities provide high-throughput, high-performance imaging of even complex experiments.

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NanoWizard V features novel scanner and sensor technologies and state-of-the-art control software that encompasses an intuitive, workflow-based graphical user interface (GUI) to ensure true, easy-to-use AFM operation.

Unmatched ease of use
Speed for dynamics and improved throughput
Automated, high pixel density mapping and imaging

NanoWizard V BioScience setup on Zeiss Axio Observer with tablet control.

From the pioneers of BioAFM with over 25 years of experience in developing BioAFMs
Recognized by an install base of over 1000 JPK/Bruker BioAFMs across the globe
Proven by 8500+ publications with biological significance
Supported by dedicated cantilever development for high-resolution imaging and customized applications

Paving the way for new scientific discoveries with:
·       PeakForce-QI, PeakForce Tapping®, PeakForce QNM®, QI
·       Single Molecule Force Spectroscopy (SMFS)
·       Single Cell Force Spectroscopy (SCFS)
·       DirectOverlay 2 for AFM in conjunction with advanced optical microscopy
·       New software environment V8
·       Features the latest ExperimentPlanner and ExperimentControl
·       Accessories for high NA optics and AFM, environment control, and more

Latest Technology for Maximum Efficiency

Highest performance BioAFM

Featuring an enhanced, workflow-based design with intuitive user guidance in the new SPM V8 software, the NanoWizard V empowers beginners and experts alike to acquire highest quality, reproducible data. The system’s high degree of automa-tion improves productivity and maximizes throughput. State-of-the-art analysis and batch processing routines ensure scientific accuracy and statistical data reliability.

Adaptive intelligent scanning routines enable faster scanning rates of up to 400 lines/sec
Lowest noise scanner and detection system ensure high-resolution data and unrivalled performance on inverted optical microscopes
PeakForce-QI, the symbiosis of PeakForce Tapping and QI modes, delivers the fastest, most advanced force control for highly delicate samples

Fastest automated BioAFM for corrugated samples on an inverted microscope

Visualize dynamics with Bruker’s Nested Scanner and latest feedback technologies
Bruker’s proven DirectDrive increases cantilever excitation stability
Active balancing provides faster scanning over large scan ranges
Improved productivity and maximized throughput enables better statistics
Intelligent optimization routines deliver quantitative nanomechanics

Most intuitive BioAFM operations

Fundamental ease-of-use features
User management, ideal for multi-user facilities
Automated setup and workflow
Single-click cantilever calibration
Single-click optical image calibration
Extended optical viewing field for AFM with stitching feature
Long-term, unattended experimental procedures
Remote operation capabilities
Optimized storage of parameters and favorites
Intuitive integration of data processing routines

Tapping mode topography image of Annexin 5 protein on lipid membrane in buffer acquired at 40 Hz line rate showing the occupation density of centric trimers in 2D honeycomb structure. Scan size 1.3 µm x 1.3 µm, height range 8.0 nm.

Fast tapping mode topography image acquired at 170 Hz line rate of Annexin 5 protein on lipid membrane in buffer. The arrows depict the occupation of a centric trimer from solution in the 2D crystal structure 12 seconds (8 frames) later. Scan size 96 nm x 96 nm, height range 1.0 nm.

Left: Topography image of atomic lattice of mica in liquid. Image taken in closed-loop on an inverted microscope, scan size 10 nm × 10 nm, height range 210 pm. Right: DNA origami (GATTA-AFM, Gattaquant, Germany) Tapping Mode topography image acquired at 400 lines/sec in TAE buffer, scan size 96 nm x 96 nm, height range 3.1 nm.

"The NanoWizard V is excellent for medical component analysis."

Prof. Dr. rer. nat. Hans Bäumler
Head of Research Department
Institute of Transfusion Medicine, Charité University Hospital Berlin (Germany)

Features

Understanding Forces in Biology

The leading BioAFM for mechanobiology

By providing the right model for the right experiment, Bruker delivers easy solutions to complex scientific questions. Automated measurement procedures allow researchers to concentrate on what’s important - their research - and to question why and not how.

Investigate single molecules, live cells, and tissue samples
Study corrugated and delicate, soft sample structures under native conditions
Gain a detailed understanding of viscoelastic properties and adhesion processes
Highest level of automation thanks to Autoalign, HybridStage or motorized stage, ExperimentPlanner, ExperimentControl, and GUI featuresfor high-speed signal processing and lowest noise levels

PeakForce-QI – a new chapter in quantitative imaging

The pioneers of quantitative biological imaging, with PeakForce Tapping (2000+ publications) and QI (~1000 publications), now bring you the next generation:
PeakForce-QI.

Fastest nanomechanical imaging thanks to latest piezo and sensor technologies
Easy, precise batch processing
True, real-time force curve monitoring

Watch the Peakforce-QI video

Correlated PeakForce-QI and fluorescence data set of live NIH-3T3 fibroblasts (murine) cells acquired at 37°C in cell medium. AFM sets blue: PeakForce-QI topography images of live fibroblasts, height ranges top 2.35 µm, bottom 3.5 µm. AFM sets red: Young’s modulus images extracted from PeakForce-QI, Z ranges top 5.2 - 13.1 kPa, bottom 0 - 30 kPa, scan sizes top 10 µm × 10 µm, bottom 23 µm × 23 µm. Overview: Overlay of fluorescently labelled fibroblasts with CellMask™ Green Actin Tracking Stain and Young’s modulus images, sample courtesy of Dr. Wedepohl, Freie Universität Berlin (Germany).

"The system’s promised speed and resolution, ease of use, and up-to-millimeter-range capabilities make this a game changer for AFM investigations in nanomedicine and bio-medical applications."

Dr. David Martinez Martin
Physicist · Senior Lecturer in Biomedical Engineering
Co-chair of the sensors and diagnostics cluster of the Nanohealth Network (University of Sydney)

Quantitative Data and Intelligent Analysis

Automated Force Spectroscopy Reloaded

The pioneers of Single-Molecule Force Spectroscopy (SMFS) and Single-Cell Force Spectroscopy (SCFS) now bring you even more flexibility, higher precision, and increased throughput.

Proven ForceRobot technology now built-in
Bruker’s intuitive and powerful RampDesigner
Automated calibration
Molecular recognition imaging
Most sensitive force control and tip-saving features

Powerful Data-Slices

Flexible creation of topography images at different forces
Proprietary contact point imaging
Image stack output of any channel from batch processing

For complex experiments, from single molecules to cells and tissues

Optimized environmental control options
Comprehensive nano-mechanics with RampDesigner
Complex experimental routines with ExperimentPlanner
Integration of several modes in long-term, unattended experiments

Unfolding of a single GB1 protein acquired in an automated, long-term experiment of ~54,000 curves in ~16 hours. Inset: Contour length difference plot. Sample courtesy of Prof. Cao, Nanjing (China).

Map of rat muscle tissue slice. Optics: Tiled 1.60 mm x 0.85 mm phase contrast and Hoechst 33342 fluorescence using HybridStage. Inset maps: Young’s modulus acquired automatically with MultiScan (z range 10 - 260 kPa). Inset plot: Loss tangent vs. frequency of two positions inside map (see arrows). Orange dots and blue squares positions 1 and 2, respectively. Sample courtesy of Prof. Ansgar Petersen, Julius Wolff Institute, Charité, Berlin (Germany).

Correlated Microscopy Newly Defined

Leading BioAFM in combination with advanced optical microscopy

Perfect integration into advanced optical techniques
Optical super-resolution (STED, FLIM, …)
Upright for tissues, implants etc.
DirectOverlay 2/MIRO
One-click optical image import
980 nm OBD option
DirectTiling, for larger scale efficiency
Stitching
MultiScan
Seamless integration of multiple optical, external and AFM channels

Background image: Fluorescence image of live E.Coli bacteria labeled with Hoechst 33342 acquired on coverslip in buffer. Middle image: Tapping Mode topography image of dividing E.Coli bacteria utilizing nested scanner technology, scan size 1.7 µm × 3.6 µm, height range 1.0 µm. Left image: Nested scanner Tapping Mode topography image zoom resolving outer membrane protein network. Scan size 200 nm × 150 nm, height range 3.5 nm.

NanoWizard V with Zeiss LSM 800 (setup courtesy of group Prof. Dr. Marie Weinhart, Freie Universität Berlin and Leibniz Universität Hannover), with BioMAT Workstation for high NA optics and Zeiss Axio Imager and on Olympus IX83 with Picoquant MicroTime 200 STED.

Optical Modes Combinations

Brightfield
DIC
Phase or modulation contrast
Confocal microscopy
Spinning disc
TIRF and IRM
FRET, FLIM, FRAP, FCS
Ca²⁺ imaging
Superresolution (STED, PALM/STORM, SIM)
Macroscope combination
Optical tweezers with OT-AFM

Unrivalled Flexibility by Design

Optimized for use in microbiology and virus research

The NanoWizard V BioAFM enables direct access to pathogens and infectious agents in a BSL facility. Each step, from sample preparation, loading and disposal to running the experiment, can be performed directly in the biosafety lab.

Suitable for working with native pathogens and biohazardous material
No “additional preparation“ steps
BSL-3 compliance achieved
(in accordance with lab’s safety regulations)

Explore an extensive range of accessories for versatile experimental setups and environmental control.

Seamless combination with the FluidFM technology enables a host of novel experiment designs and applications.

DMPC ripple phase formation imaged in Tapping Mode at 200 Hz line rate in EDTA buffer using the nested scanner feature. The sequence shows two different ripple phase formations with increasing/decreasing temperature. Scan size 300 nm × 300 nm, z range 5 degrees.

P(NIPAM-NEAM) hydrogel microparticles for cell-interactive scaffold engineering analyzed with QI at varying temperatures in the BioCell. The images demonstrate the QI topography and Young’s modulus map on the particle and a series of optical phase contrast images.

The top image shows the corresponding QI force curve., the bottom curve shows the increase in Young’s modulus (blue) and decrease in size (orange) with increasing temperature. Sample courtesy of Prof. De Laporte, Aachen (Germany).

Applications

Life Science AFM Data Gallery

Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.

Browse the full gallery here

Specifications

Operating Modes

Standard Operating Modes

Now with PeakForce-QI including PeakForce Tapping, QI and PeakForce QNM
Including fast PeakForce Tapping and QI with nested scanner technology
Contact mode with lateral force microscopy (LFM)
Tapping Mode™ with PhaseImaging™
ExperimentPlanner for designing a specific measurement workflow
Static and dynamic force spectroscopy
Advanced Force Mapping

Optional Modes

Advanced spectroscopy modes such as various force clamp modes or ramp designs
Fast scanning option with line rates of up to 200 Hz
QI Advanced mode for quantitative data, perfect for soft samples
ScanAsyst automated gain and setpoint adjustment in PeakForce Tapping and PeakForce-QI

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Advanced AC modes such as FM and PM with Q-control & Active Gain Control
Microrheology in CellMech Package
Kelvin Probe Microscopy
MFM and EFM
Conductive AFM
STM
Electrical spectroscopy modes
Piezoresponse Microscopy for high voltages
Electrochemistry & Scanning Electrochemistry with temperature control and optical microscopy
NanoLithography and NanoManipulation
NanoIndentation
Scanning Thermal AFM
FluidFM® solution from Cytosurge
ExperimentControl feature for remote experiment control
DirectOverlay 2 for combined AFM and optical microscopy
Additional XY or Z sample movement stages available with CellHesion®, TAO™ and HybridStage™ module

Accessories

The Widest Range of Accessories in the Market

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.

Browse NanoWizard V BioScience AFM Accessories

Or Visit Our Online AFM Probes Store

Webinars

Watch Recent BioAFM Webinars

Our webinars cover best practices, introduce new products, provide quick solutions to tricky questions, and offer ideas for new applications, modes, or techniques.