JPK NanoWizard 4 XP BioScience

NanoWizard® 4 XP BioScience

The NanoWizard 4 XP BioScience atomic force microscope combines atomic resolution and fast scanning with rates of up to 150 lines/sec and a large scan range of 100µm in one system. It is designed to provide highest mechanical and thermal stability on inverted optical microscopes during long term experiments on samples ranging from single molecules to living cells and tissues.

Performance

150 lines/sec and 100 µm scan range

Understand the complexity of biological systems: Trigger events via precise control of temperature, pH, and fluid/gas exchange. Observe response to stimuli and sample dynamics.

High Resolution

Quantitative Imaging

Structural analysis and nanomechanical characterization of biological samples. Precise investigation of molecular mechanisms, biochemical interactions, and cell mechanics.

Flexibility

Integration with advanced optical microscopy

Simultaneous, correlated measurements with techniques like FRET, TIRF, FLIM, FCS, epifluorescence, confocal laser scanning and STED microscopy.

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Features

High-resolution imaging with extreme performance

The NanoWizard 4 XP BioScience comes with:

PeakForce Tapping® for easy imaging
Fast Scanning option with up to 150 lines/sec
NestedScanner Technology for high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
New tiling functionality for automated mapping of large sample areas
V7 Software with revolutionary new workflow-based user interface
DirectOverlay™ 2 software for perfect integration and data correlation with advanced fluorescence microscopy platforms
Vortis™ 2 controller for high-speed signal processing and lowest noise levels

Tailored DNA origami frames imaged in TAE 10mM MgCl2 buffer on mica. Sample courtesy of R. Willaert, VUB, Brussels (BE). Scan field: 125nm · Height range: 4.4nm · Scan speed: 150 lines/sec

Automated mapping of large sample areas with new tiling functionality

Living Vero cells in cell culture medium at 37°C.

The HybridStage™ or Motorized Precision Stage transforms experiments by enabling direct access to a large sample area, with automated, motorized movement to selected positions, grids and mapping regions.

Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.

Precise motor movements automatically bring the whole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.

The images show living Vero cells in cell culture medium at 37°C in the PetriDishHeater™ . [2] - [5] Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. - Zoom into region scanned with AFM showing 100μm×100μm scan (height range 5μm) and inset 15μm×15μm (height range 2μm) scan topography images using PeakForce Tapping. The feedback correction signal images highlight the surface membrane features, particularly in the zoomed image. Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary.

Perfect optical integration provides true correlative microscopy

The NanoWizard 4 XP, with its unique tip-scanning technology and fast imaging capabilities, is ideal for taking advantage of the synergy between AFM and super-resolution microscopy.

The NanoWizard 4 XP is compatible with a wide range of platforms, such as those from Zeiss (PALM/STORM, SIM), Leica (STED), PicoQuant (STED), Nikon (SIM, STORM) and Abberior (STED).

The 980nm laser option for the AFM head allows the simultaneous use of optical microscopes and focus stabilization systems, critical for long term experiments, and avoids conflicts with fluorescence or spectroscopy measurements.

STED and AFM experiment of living A549 cells imaged at 37°C in medium. [1] STED image of microtubules labelled with silicon rhodamine overlayed with AFM topography. [2] AFM QI topography image at 240pN imaging force (height range 3.5μm). [3] Corresponding QI Young‘s modulus image (z range 100kPa).

The images show NanoWizard 4 XP setups on Zeiss LSM 880 confocal microscope with Airyscan [1] , with Upright Fluorescence Microscope (UFM) Kit for tissues or other large samples such as organs [2] , with BioMAT Workstation for high NA optics and Zeiss Axio Imager [3] , with TopviewOptics [4] and on on Olympus with PicoQuant MicroTime 200 STED [5].

Outstanding quantitative data from molecules, cells and tissues

QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).

The image shows single cell force spectroscopy measurements using the CellHesion module with an increased z range of 100 µm, showing the detachment force curves of a single A549 cell from fibronectin (FN) and from bovine serum albumin (BSA) coated culture dishes. Note, that the detachment of the cell from fibronectin results in a very large pulling range of 77 µm.

Single cell force spectroscopy measurements using the CellHesion module.

[1]-[2] Stiffness mapping of non-cancerous human cervix tissue with a HybridStage. The inset in the fluorescently-labelled (Hoechst) slab was used for mapping of a 5×4 quadrant area of 1000μm×800μm with overlayed composite Young‘s modulus map shown in the middle panel. [3] The representative topography channel from an individual 200μm×200μm channel is given in the right panel. Sample courtesy of Dr. T. Fuhs and Prof. J.A. Käs, University of Leipzig, Germany.

Key Features

Fast Scanning option with up to 150 lines/sec for tracking dynamic processes
Now with Bruker’s exclusive PeakForce Tapping as standard
Large scan field of 100 × 100 × 15 µm³ and atomic lattice resolution on inverted microscopes
New workflow-based user interface for ergonomics and ease of operation
New tiling functionality for automated mapping of large sample areas together with the HybridStage
Enhanced DirectOverlay 2 mode for most precise correlative microscopy
New Vortis 2 controller with high-speed low-noise DACs and cutting-edge position sensor readout technology
Highest flexibility and upgradeability with a broad range of modes and accessories

NanoWizard 4 XP BioScience setup on Zeiss Axio Observer with new user interface and tablet control.

Applications

NanoWizard 4 XP BioScience Data Gallery

Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.

Browse the full gallery here

Atomic lattice of mica in buffer, taken in closed-loop on an inverted microscope.

Reorganization of cytoskeletal actin in the form of transverse arcs and radial fibers during the filopodia formation of living fibroblasts at 37°C (top). Time-lapse of consecutive phase images (taken 15 s apart) allows the identification of individual dynamic events, such as vesiculation and translocation of cargo molecules (bottom). Sample courtesy of Prof. A. Hermann, Humboldt University, Berlin.

Individual DNA molecules on PLO covered mica, imaged in liquid (height range 2.6nm). Major and minor grooves visible at various locations, e.g., inset shows helical repeat of 3.5nm and 3.6nm, respectively.

Topography image of bacteriorhodopsin in buffer. Height range 600pm.

Fixed mouse cerebellum tissue embedded in 4 % Agarose. BioMAT Workstation was used to overlay [1] 63× upright fluorescent microscopy image of DAPI stained nucleus and [2] AFM force mapping height (z range: 30 µm) and [3] Young´s modulus image (z range: 5 kPa, scan size: 50 µm). Newly developed automatic height compensation was used to overcome the typically large height differences of tissue samples. Sample courtesy: AG Prof. Jochen Guck, Dr. Elke Ulbricht, TU Dresden, Germany.

Specifications

Operating Modes

Standard Operating Modes

Imaging modes

Now with PeakForce Tapping
Contact mode with lateral force microscopy (LFM)
Tapping Mode™ with PhaseImaging™

Force measurements

Static and dynamic spectroscopy
Advanced force mapping

Optional Modes

Fast Scanning option with up to 150 lines/sec
Fast QI Advanced mode for quantitative data, perfect for soft samples
Mechanical properties such as adhesion, elasticity, stiffness, deformation
Conductivity and charge distribution mapping
Contact Point Imaging (CPI) with zero force
Molecular recognition imaging for binding site mapping
Advanced AC modes such as FM and PM with Q-control & Active
Gain Control

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Higher harmonics imaging
Kelvin Probe Microscopy and SCM MFM and EFM (see also QI mode)
Conductive AFM (see also QI mode)
STM
Electrical spectroscopy modes
Piezoresponse Microscopy for high voltages
Electrochemistry with temperature control and optical microscopy
NanoLithography
NanoManipulation
Nanoindentation
Scanning Thermal AFM
FluidFM® solution from Cytosurge
ExperimentPlanner for designing a specific measurement workflow
RampDesigner™ for custom designed clamp and ramp experiments
ExperimentControl feature for remote experiment control
DirectOverlay 2 for combined AFM and optical microscopy
Additional XY or Z sample movement stages available with CellHesion®, TAO™ and HybridStage™ module

Accessories

The Widest Range of Accessories in the Market

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.

Browse NanoWizard 4 XP BioScience Accessories

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Software

New workflow-based user interface redefines user-friendliness

The new V7 software interface guides users through the workflow to set up experiments intuitively and makes it simple, even for users with minimal AFM experience, to progress confidently to generating high-quality data. Each stage of the setup and operation works as an optimized desktop that brings all the vital information into focus with a single click.

On-screen, context-sensitive help
Status feedback for alignment and setup
Efficient task-based experiment selection
Fast access to favorite and recently used experiments
One-click probe calibration
Instant overview of the key data
User management for Multi-User environments like imaging facilities

Webinars

Watch Recent BioAFM Webinars

Our webinars cover best practices, introduce new products, provide quick solutions to tricky questions, and offer ideas for new applications, modes, or techniques.