InVi SPIM Lattice Pro
InVi SPIM Lattice Pro
The InVi SPIM Lattice Pro provides the highest level of flexibility for illuminating samples using a proprietary Advanced Illumination Module (AIM). It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.
Illumination and Detection
The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices.
The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs.
InVi SPIM Lattice Pro Design
Live Sample Applications
Browse a selection of applications data from our customers below. Researchers are using the InVi SPIM Lattice Pro in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
Mitosis in HeLa Cells
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
Imaged on the InVi SPIM Lattice Pro.
Visualization: Imaris (Bitplane).
Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
3D Imaging of a Spheroid
Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.
Courtesy of:
Martin Stöckl
University of Konstanz
Germany
HeLa Cell Culture
HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.
Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands
Specifications
| Illumination | Detection |
Effective Magnification |
Field of View | Pixel Size | Optical Resolution |
| 28.6x / 0.7 NA | Nikon 25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
Illumination Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation with static illumination or by beam scanning
- Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
- Special Optics 28.6x 0.7 NA water immersion objective lens
Detection Optics
- Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
- 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
System Comparison
* COMPATIBLE MODULE
| MuVi SPIM Multiview |
InVi SPIM Lattice Pro Inverted View |
LCS SPIM Large Cleared Samples |
TruLive3D Imager |
|
|---|---|---|---|---|
| Benchtop Design |
✓ | ✓ | ✓ | ✓ |
| Configuration |
Horizontal Multiple-View |
Inverted |
Inverted - Dual Illumination |
Inverted - Dual Illumination |
| # Lenses |
4 lenses (2 IO, 2 DO) |
2 lenses (1 IO, 1 DO) | 3 lenses (2 IO, 1 DO) | |
| Multi-View |
✓ | |||
| Live/Fixed Samples |
✓ | ✓ | ✓ | |
| Cleared Samples |
✓ | ✓ | ||
| Best Embedding |
Capillary/FEP tube w/ agarose 3D stage |
FEP foil; glass slides |
Quartz-crystal cuvette | TruLive3D dishes and FEP foil |
| Photomanipulation* |
✓ | ✓ | ✓ | |
| Environmental Control* | ✓ | ✓ | ✓ | |
| Destriping/Uniform Illumination* | ✓ | ✓ (w/ Advanced Illumination Module) | ✓ | ✓ |
All-in-One LuxBundle Software
Intuitive design
Bruker's LuxBundle software saves time and enhances productivity by providing:
- All-in-one, easy-to-use interface for acquisition, viewing, and post-processing
- Fully scriptable microscope control and post-processing via open interface (e.g., Python or any other language) ready for custom "smart" microscopy
- High reproducibility of experiments: all parameters are saved in the metadata and configurations can be saved for future experiments
- Data formats (.tiff, .hdf5, .ims) compatible with common image processing software: Imaris, Aivia, BigDataViewer, Arivis, Fiji, Python, Matlab, Napari
Impressive Image Post Processor
MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxBundle software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
- Multi-color alignment
- Tile stitching of hundreds of tiles for large samples
- Multi-view image fusion and deconvolution
3D Data Viewer
LuxBundle's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including:
- The ability to turn tile stitching on or off
- Both raw and post-processed images
- Fast viewing of multi-terabyte data sets
- Flexible options to draw and annotate regions and landmarks
