Overview
This course is designed for novice users of the Bruker D8 QUEST or D8 VENTURE platform for X-ray crystal structure determination with a focus on biological crystallography. Each workshop offers basic training in crystallography as well as hands-on instrument training covering both hard and software. Instrument time, reference samples and IT infrastructure will be provided. Introduction to protein preparation, characterization and crystallization.
- Introduction to single crystal diffraction (crystal systems, space groups, X-ray diffraction theory
- Overview of instrument hardware and software
- Techniques for selection and mounting of specimens for X-ray analysis
- Data collection at room temperature using the in situ ISX stage
- Discussion of data collection techniques and strategies
- Hands-on use of a Bruker D8 series system for routine structure determinations
- Instruction and best practice with Proteum5 software package
- Import/Export/Conversion of data with Proteum5
- Structure determination and refinement - use of CCP4 software package
- De novo structure solution by SAD using the SHELX suite
Further Information
Location
Language
English
Class size
Training should not precede the installation of the system concerned.
Class sizes are limited, so that each participant receives individual attention and participates in all hands-on activities. Early registration is encouraged as registrations are accepted on a first-come/first-served basis.
Attendance fee
For courses taking place at our Karlsruhe facility, the course price includes course materials, lunch, and refreshments. Travel and accommodation are not included.
For remote courses, the fee includes 1 hour of remote follow-up support (to be used within 1 month after completion of the course).
Cancellation
Registrations cancelled within 10 working days prior to the start of the course are subject to a cancellation fee of 30 %. Registered persons not attending the course without having given prior notice will be fully charged. We reserve the right to cancel a course if there are less than three registrants by the registration deadline.
Contact
For further information about the course content, please contact:
Tobias Stürzer
Application Scientist SC-XRD
For information about price, registration, or other questions:
Evi Breitinger
For a quotation, please contact: