Putting Molecules in Place Using Correlated Light-Sheet Microscopy and X-Ray Tomography
Correlated Light-Sheet Microscopy and X-ray Tomography for Mapping Gene Expression Patterns
In this webinar, Dr. Venera Weinhardt speaks about using correlated LSFM and X-ray computed tomography approaches in applications, such as mapping gene expression patterns within the 3D anatomy of whole organisms at cellular resolution. The following topics are covered in this webinar:
- Principles of multimodal imaging to map both function and structure within organisms
- Multimodal imaging pipeline and 3D data processing
- Using visual and quantitative insights into the 3D distribution of gene expression within tissue architecture as reference atlases for model organisms’ development and their malformations
- Using multimodal approaches to identify tissues together with their 3D cellular and molecular composition in anatomically less-defined in vitro models, such as organoids.
Presenter's Abstract
The development of an organism is orchestrated by the spatial and temporal expression of genes. Accurate visualization of gene expression patterns in the context of the surrounding tissues is a prerequisite to understanding the mechanisms that drive morphogenesis. In this webinar, I will show correlative light-sheet fluorescence microscopy and X-ray computed tomography approaches to map gene expression patterns to the whole organism’s 3D anatomy at cellular resolution. This multimodal approach applies to gene expression visualized by protein-specific antibodies and fluorescence RNA in situ hybridisation offering a detailed understanding of individual phenotypic variations in model organisms. The approach offers a unique possibility to identify tissues together with their 3D cellular and molecular composition in anatomically less-defined in vitro models, such as organoids. The visual and quantitative insights into the 3D distribution of gene expression within tissue architecture, by multimodal approach developed here, will be equally valuable for reference atlases of model organisms development, as well as for comprehensive screens, and morphogenesis studies of in vitro models.
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Guest Speaker
Venera Weinhard, Ph.D., Group leader, Centre for the Organismal Studies, University of Heidelberg
Venera Weinhardt, a physicist specializing in novel imaging techniques for life sciences, excels in developing and adapting methods such as new contrast mechanisms, in vivo/low-dose imaging, and innovative geometries. Passionate about deploying imaging technologies effectively, she focuses on solutions for both laboratory and tabletop systems. Leading an interdisciplinary team, she engages in teaching physics, biology, and computer sciences. Venera Weinhardt has received several awards including the Walter Benjamin Program, Marie Skłodowska-Curie Actions, and EU Research and Innovation Act. Beyond her work, she indulges in art classes, tennis, and reading.