Discover the fascinating capabilities of combining Bruker’s BioAFM techniques with the Cytosurge FluidFM technology.
Featuring special guest speakers alongside a panel of Bruker application experts from across Europe, the program includes both detailed presentations and live demonstrations highlighting the features, capabilities, and practical use of these tools. Attendees can expect to gain new insight into the novel experimental designs and applications made possible by combining FluidFM with Bruker’s BioAFMs.
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Combining Atomic Force Microscopy and Nanofluidics
How FluidFM technology enhances the versatility of Bruker BioAFMs
Atomic Force Microscopy (AFM) is an advanced multi-parametric imaging technique that enables the 3D imaging of the topography of biological samples in the nm-range, the characterization of their nanomechanical properties, and the visualization of interactions and structural changes occurring at the molecular level.
The FluidFM (Fluidic Force Microscopy) technology features microchanneled, force-sensing cantilevers with microfluidic pressure control. They enable the handling of femtoliter volumes, precise pressure control (0.1mBar), and can be used as nano-syringes to extract cell material or inject fluids into singles cells or tissues.
The seamless combination of FluidFM with Bruker’s BioAFMs opens the door to a host of novel experiment designs and applications. It enables a broader range of force control, the use of microfluidics for single-cell manipulation applications, and accurate positioning combined with perfect optical integration for observation and control.
Applications include:
- Single-cell injection or extraction
- Single-cell level adhesion measurements with forces >50nN
- Enhanced nanomanipulation capabilities
- Trigger-Tracking experiments: Precise localized injection into cells (FluidFM) and tracking of interactive forces, topographic changes, or nanomechanical properties (AFM)
- Access to single-cell gene editing, injection of targeted drugs or gene vectors for AFM users
Featured Presentations
Mirko Vanetti, Ph.D., Cytosurge AG, Switzerland
Dr. Mirko Vanetti, Cytosurge AG, will speak on the growing field of applications for the FluidFM technology, and outline examples of how the technology can support CRISPR, neurobiology, and virus research.
Torsten Mueller, Ph.D., Senior Developer Life Sciences, Bruker
André Körnig, Ph.D., Applications Scientist, Bruker
André Körnig, Ph.D., Applications Scientist, Bruker
Speakers
With introduction and closing remarks by Carmen Pettersson, Marcom Manager EMEA, Bruker BioAFM
Cécile Formosa-Dague, Ph.D., CNRS Researcher, Toulouse Biotechnology Institute (TBI), INSA de Toulouse, France
Dr. Cécile Formosa-Dague did her PhD between 2012 and 2015 at the Laboratory for Analysis and Architecture of the Systems (LAAS-CNRS) in Toulouse, where she worked on important issues related to multidrug-resistant microorganisms using AFM. After that, she joined the Y. Dufrêne team at the Catholic University of Louvain (UCL) in Belgium, where she pushed further the technological concepts developed during her PhD to study biofilm formation in bacteria. In 2017, after having won a Marie Curie grant, she moved to the TBI (Toulouse Biotechnology Institute) where she worked on developing interdisciplinary approaches to studying microalgae and their interactions with their environment using AFM techniques. Finally, in 2019, she obtained a permanent position at the Centre National de la Recherche Scientifique (CNRS) where she continues to develop these research activities on microalgae, at the TBI, and to work on new techniques based on the FluidFM technology.
Mirko Vanetti, Ph.D., Cytosurge AG, Switzerland
