Human Metabolomic Research - Insights

Explore the clinical research impact of NMR-based methodologies with Juan Manuel Lopez Smith. In this informative video, Juan Manuel discusses how advanced NMR spectroscopy is being applied in the context of tuberculosis and related drug resistance. Learn about the innovative assays developed to determine drug resistance in Mycobacterium tuberculosis and the potential of benchtop NMR equipment to make these techniques more accessible in clinical settings. Discover how these advancements are contributing to human metabolomics and addressing critical medical challenges. Please note that the methodologies presented are for research use only and are not applicable for diagnostic procedures.

In this interview, Prof. Patrick Giraudeau from Nantes University shares insights into his work with NMR spectroscopy in human metabolomics. He emphasizes that NMR spectroscopy stands out as an unparalleled tool for analyzing complex mixtures like body fluids because of its unique capacity to provide both structural and quantitative data with high reproducibility. Prof. Giraudeau's work focuses on fast 2D NMR methods and hyperpolarized NMR techniques and seeks to push NMR’s boundaries, enhancing its performance to reveal previously inaccessible information within complex mixture samples. Prof. Giraudeau also discusses his excitement about the upcoming installation of the standardized IVDr platform, known for its reproducibility, stability, and reliability, which opens opportunities for inter-laboratory studies and collaborations with other facilities equipped with the same technology. Lastly, he highlights the promising role of benchtop NMR systems in personalized healthcare.

Main research topics at the laboratory for Metabolomics and Systems Medicine at the Werner Siemens
Imaging Center & University of Tübingen

• Quantitative IVDr-NMR spectroscopy of human biofluids (serum/plasma, urine, liquor)
• Quantitative NMR spectroscopy of murine biofluids (serum, urine)
• Tissue metabolomics and lipidomics with human and murine samples (e.g. liver, brain, kidney)
• Cell culture and feces/stool metabolomics and lipidomics including multi-omics studies
• Biomarker discovery and personalized precision medicine

As highly interdisciplinary facility, the Werner Siemens Imaging Center is dealing with many studies within neurology, oncology, inflammation and more. Hereby, the laboratory for Metabolomics and Systems Medicine measures and analyses large-cohort samples from preclinical and clinical studies (e.g. Parkinson’s and Alzheimer’s disease, breast cancer, brain tumors, (post) Covid-19 syndrome, cardiovascular disease, rare diseases, (pre)diabetes and many more) upon their metabolite concentrations in blood, urine, liquor, feces or tissue and puts them together with their collaboration partners into a systems biological context.
 

For more Info about the Metabolomics Cancer Research at Uni Tübingen also watch this: 

• NMR studies of protein-carbohydrate interactions (monoclonal antibodies, lectins, selectins, siglecs, glycosyltransferases)
  
• NMR studies of ligand binding to native viruses and viral coat proteins. Design of novel entry inhibitors
• Recombinant production of isotope labeled glycosyltransferases for NMR spectroscopic experiments
• Studies of thermodynamic and kinetic aspects of carbohydrate-protein recognition reactions using NMR, surface plasmon resonance and microcalorimetry
• Structural and biophysical characterization of skin matrix proteins involved in autoimmunity

Bruker NMR Instruments are for Research Use only and are not intended for Use in Clinical Diagnostic Procedures.