Biologics Higher Order Structure

The latest generation of NMR instrumentation, combined with newly developed NMR acquisition methods, means that intact molecules, including antibodies (150 kDa), can be characterized at natural abundance, in formulation buffer or other physiologically relevant conditions. Differences between batches are detected by comparing the spectrum of a batch with a reference, thus allowing rapid pass/fail decisions to be made.   

 

Easy acquisition: The latest generation of NMR instrumentation affords high spectral resolution and high sensitivity. When combined with newly developed NMR acquisition methods, this enables the study of intact molecules, including antibodies (150 kDa) at natural abundance in formulation buffer or other physiologically relevant conditions.

Easy analysis: Easy-to-use NMR analysis software also makes this solution accessible to non-NMR experts.

• Similarity assessment: Published & accepted methods are available
  o    PROFILE 1D
  o    PCA – unsupervised chemometrics
  o    Multivariate statistic model building and distance calculations
  o    ECHOS – simple representation of spectral differences
  o    CCSD – represents shifts in peak positions.
• Matrix analysis for the comparison of batches
• Integrated solution: Data and results are linked, enabling the reason(s) for any observed spectral differences to be determinated.
  
  

Mnova BioHOS software features

• Easy-to-use NMR analysis software makes this solution accessible to non-NMR experts
  
• Similarity assessment. Published & accepted methods are available
  - PROFILE 1D
  - PCA – unsupervised chemometrics
  - Multivariate statistic model building and distance calculations
  - ECHOS – simple representation of spectral differences
  - CCSD – represents shifts in peak positions
• Matrix analysis for the comparison of batches
• Integrated solution: data and results are linked, giving insight on the potential reason(s) for observed spectral differences.