ELEXSYS-II E580

Multifrequency

The E580 employs an IF (Intermediate Frequency) design that facilitates easy upgrades from the standard X Band to L, S, and Q Bands

0.5 ns

The SpecJet-III digitizer and VAMP-III video amplifier offer a very wide detection bandwidth of 1 GHz.

Shaped Pulses

The SpinJet AWG (Arbitrary Waveform Generator) unlocks the world of shaped pulses for improved sensitivity and selectivity.

ELEXSYS E580, the ultimate EPR System for Life Science, Materials Science and Quantum Computing

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Features

FT-EPR and Electron Spin Echo techniques as the basic detectors for the EPR spectrum and electron spin relaxation times (T₁ and T₂)
ESEEM and 2D-HYSCORE to measure spin density distribution and distances through the hyperfine interaction between electron and nuclear spins
Pulse-ENDOR to measure hyperfine interactions and nuclear spin relaxation
Pulse-ELDOR and DEER to measure long range distances by electron-electron spin dipolar coupling
SECSY and EXSY to measure correlations and exchange rates
Laser triggered experiments to investigate chemical reactions and triplet states

SECSY and EXSY

Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques. The positions of cross peaks in the 2D spectrum are used to correlate spectral peaks with each other and the cross peak amplitudes carry the information about exchange rates. These experiments are standard features of the E 580 spectrometer and are supported by predefined PulseSPEL programs.

Laser Triggered Experiments

An external event, e.g. a laser flash, can be used to generate a transient paramagnetic state. For this type of experiment the instrument can be triggered externally or alternatively the instrument can send a trigger pulse to activate an external device. Precise synchronization between the external event, the microwave pulses and the data acquisition is accomplished. Any pulse-EPR experiment can be run in combination with the external trigger function.

Pulse ELDOR and DEER

Electron-Electron Double Resonance (ELDOR) and Double Electron-Electron Resonance (DEER) are acronyms for the same technique. With this technique the dipolar interaction between electron spins is measured. This information directly translates into the distance between the electron spins. Distance ranges of 1 - 8 nm are covered. In recent years the combination of site directed spin labeling and DEER became one of the most popular tools in structural protein research.

ESEEM and 2D-HYSCORE

Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins. From the analysis of the ESEEM signals detailed information about electron spin density distribution, distances and bonding angles is gained, offering valuable structural and electronic information. 2D-HYSCORE is a technique also based on the ESEEM effect and is the method of choice for frozen solutions and powders. All ESEEM-type of experiments are standard features of the E 580 and are supported by dedicated acquisition and processing tools in Xepr.

1H HYSCORE of nitroxide spin probe: (right) 166 ns Chirped (± 113 MHz) inversion pulse and (left) 26 ns standard inversion pulse. The excitation bandwidth of the inversion pulses are indicated on the field sweep spectrum, red = chirped and blue = standard.

4-Pulse DEER spectra of spin-labeled Cu containing protein with the pump pulse centered on the nitroxide spectrum (red arrow) and observation in the Cu spectrum (blue arrow). Sample courtesy Thomas Prisner

Pulse ENDOR, a complementary technique to ESEEM, combines both MW and RF pulses. It measures hyperfine interactions (HFI) to give detailed information about electron spin density, distances, and bonding angles. It excels at strong HFI that occur for very short distances or transition metal nuclei.

Substantial increase of sensitivity in DEER experiment at Q‑Band (33.5 GHz, ~25 min measuring time) as compared to X‑Band (9.5 GHz, ~22 h measuring time).

Measurement of the electronic relaxation rates, T₁ and T₂, offers insights into dynamics and structure. T₁ supplies information regarding molecular collisions, excited state coupling, lattice vibrations, and distances between paramagnetic centers. T₂ gives a wealth of information about molecular tumbling, internal rotations, dipolar interactions and distances.

Specifications

Pulse EPR has matured into a well established tool for the investigation of paramagnetic species. From the very beginning Bruker set the milestones in commercial Pulse-EPR instrumentation with the ESP 380 introduced in 1987. The next landmark was set by the successor model ELEXSYS E 580 and the new high speed digital devices PatternJet and SpecJet. For the first time in history it was possible to average FIDs and echoes at a rate governed by spin relaxation and not hampered by hardware constraints.

Accessories

Another cornerstone of the spectrometer, the probehead, has been in the focus of development over many years. The result is the legendary Flexline probehead series.

The ELEXSYS E 580 FT/CW X-Band spectrometer is also the platform for multi-frequency extensions. Based on the X-Band spectrometer, frequency conversions are available to W-Band (E 680), Q-Band (SuperQ-FT), S-Band, (SuperS-FT) and L-Band (SuperL-FT). The unique design concept allows transformation of all features of the X-Band to the additional frequency in a dual-band spectrometer.

Software

Xepr for experiment design and data handling

Unprecedented flexibility and ease of use are the attributes of the Xepr software. Whether you are dealing with a simple echo decay or a complicated multiple-pulse 2D experiment, the graphical user interface of Xepr ensures easy instrument control, experiment definition and execution. Numerous novel processing tools, display capabilities and data representations open up new dimensions in data analysis as well.

Webinars

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LabScape

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory