The JPK NanoWizard® ULTRA Speed 2 delivers exceptional performance and unmatched user-friendliness. It reaches speed levels previously unattainable with traditional AFMs and combines true atomic resolution and fastest scanning with rates of 10 frames/sec. Real-time, in-situ experiments can be performed in combination with advanced optics. A broad range of modes and accessories makes the system highly flexible and upgradable.
The NanoWizard ULTRA Speed 2 provides a range of new features:
The images show Plasmid-DNA with major and minor grooves imaged in liquid (top) and true atomic resolution of a calcite crystal plane in liquid recorded at 100 lines/sec (bottom).
The system comes with the lowest noise and highest stability available on the market to provide true atomic resolution. Direct force control at ultra-low forces prevents damage to your samples and probes. With the state-of-the-art position sensor technology, the system delivers highest accuracy and maximum precision.
The video shows AFM images of the same individual DNA molecule acquired in liquid over 400 consecutive scans, which demonstrate the low invasiveness and stability (rate 10 frames/sec). With conventional AFM (4 lines/sec), this experiment would take more than 2 hours.
Until now, performing dynamic experiments on living cells, highly corrugated samples or steep surface structures with highest spatial and temporal resolution was challenging. With our new NestedScanner technology, cells, bacteria or structured surfaces with samples heights up to 8µm can now be examined at the highest scan speeds.
The video shows melting and cystallization of a thin film of biodegradable Polyester polycaprolactone (PCL) by ramping the sample temperature from 33°C to 62°C and back while scanning. The NestedScanner technology allows high speed scanning of 225µm/s during the entire temperature cycle by following the swelling and contraction of the PCL film over the whole height range of 2.5 µm.
Peak Force Tapping enables even inexperienced users to precisely control probe-to-sample interactions and minimize imaging forces. This is vitally important for soft, and fragile biological samples.
This superior force control results in the most consistent, highest resolution AFM imaging, and is suitable for the widest range of sample types.
Using PeakForce Tapping, crystal-clear images can be obtained with just a few clicks, and without any expert knowledge or cantilever tuning necessary. Never before has it been so easy to image a sample.
The image shows Peakforce Tapping on DNA Origami (GATTAAFM, Gattaquant, Germany) on mica in TAE buffer.
Enhance your system with the HybridStage to perform automated mapping of large large samples areas. The HybridStage™ frees experiments from the constraints of the AFM piezo range. Large-range tiling of optical images provides a clear visual overview, allowing a fast setup of optically guided experiments and direct selection of the optical features for investigation.
Navigate around the sample, collect a list of interesting features for Multiscan, or even map force responses over greatly extended scan ranges.
The HybridStage is a newly developed modular, piezobased sample scanner stage combined with motorized XY sample movement, giving direct access to anything you can see. The highly versatile solution for all your sample requirements.
The images show living Vero cells in cell culture medium at 37°C in PetriDishHeater™ . [1] Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. [2] Zoom of the regions indicated and multiple AFM images using PeakForce Tapping mode. [3] Height image of region (a) in (2) with pixel difference filter applied [4] Height image of region (b) in (2) showing microvilli, z range 500nm; Sample courtesy of Prof. A. Herrmann, Humboldt University, Berlin
The new V7 software interface guides users through the workflow to set up experiments intuitively and makes it simple, even for users with minimal AFM experience, to progress confidently to generating high-quality data. Each stage of the setup and operation works as an optimized desktop that brings all the vital information into focus with a single click.
The combination of AFM with the Nobel prize-winning super-resolution technologies (STED, PALM/ STORM) provides enhanced imaging capabilities.
The NanoWizard ULTRA Speed 2, with its unique tip-scanning technology, can also be integrated with single molecule techniques like FRET, FCS, FLIM, TIRF to provide additional optical data sets when performing dynamic experiments on living cells or molecules. Correlation with other advanced optical techniques like confocal, spinning disc, and structured illumination techniques (SIM), combined with livie cell imaging, makes this system the perfect choice for applications in medical, biophysical, chemical or material research.
The newly enhanced DirectOverlay 2 software module enables the direct correlation of AFM and optical data. The capabilities of our calibration algorithms, visualization routines and usability have all been expanded to provide the most user-friendly functionality available today.
The images show a correlative Experiment of AFM and STED on ATTO-647N labelled DNA nanorulers in TAE-buffer. Sample courtesy: GATTAQUANT GmbH, Germany [1] and correlative STED and AFM images of isolated sacculi of Bacillus subtilis with cell division protein (FtsZ) labeled for highlighting z rings. Sample courtesy: R.K. Tank1,3 · R.D. Turner2,3 · S. Kumar1,3 N. Mullin1,3 · A. Cadby1,3 · S.J. Foster2,3 · J.K. Hobbs1,3; 1 Department of Physics and Astronomy · 2 Department of Molecular Biology and Biotechnology · 3 The Krebs Institute; all University of Sheffield, UK [2].
The new, high-speed, low-noise Vortis 2 controller is a brilliant piece of precision engineering. With more processing and computing power than any other controller today, Vortis 2 sets the standard. Equipped with the latest FPGA technology and a dual core Power PC, the system can process a huge amount of data in the shortest timeframe. Vortis 2 comes with faster, low-noise DACs and a cutting-edge position sensor readout technology. The system offers a large number of feedback modes, a powerful HV amplifier module for high speed applications and is passively cooled to keep acoustic noise in the lab low.
The new system comes with more accessories and modes than any other AFM platform, each specifically developed to suit the broadest range of applications.
Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.
Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.
Our webinars cover best practices, introduce new products, provide quick solutions to tricky questions, and offer ideas for new applications, modes, or techniques.