Knowledge Pack: Super-Resolution Microscopy

This protocol can be used to prepare imaging buffer for super-resolution dSTORM imaging applications.

This protocol can be used to fix cytoskeleton-tubulin in cells for super-resolution microscopy applications.

This protocol can be used to prepare cell culture samples for super-resolution microscopy applications.

       _{PRESENTATION HIGHLIGHTS:}

• [00:00:00] Introduction & Overview
• [00:02:53] Why Super-Resolution?
• [00:05:09] Localization Microscopy Methodology
• [00:13:26] Key Properties of Photoswitching
• [00:20:15] Dye Selections for Distinct Multicolor Imaging
• [00:23:34] SMLM Workflow: Sample Preparation
• [00:43:26] SMLM Workflow: Acquisition
• [00:53:20] Q&A