Advanced Multilayered Solutions for Successful Calcium Imaging in Neuroscience

       _{PRESENTATION HIGHLIGHTS:}

• [00:00:00] Introduction to the technology of miniscopes and to the new nVue Platform.
• [00:02:35] Streamlined and completed Workflow Solution for Free Behaving Imaging and Analysis, a step-by-step guide.

Imaging Examples using the nVue Platform

• [00:04:20] Calcium Imaging using preview integrated lens and RTI
• [00:06:20] Calcium Imaging using animal models that naturally express GCaM
• [00:07:40] Imaging deeper with more signal

The Different types of layering possible with nVue

• [00:08:59] Simultaneous GCaMP & GRAB-DA imaging
• [00:10:12] Simultaneous GCaMP & DA-sensor imaging
• [00:11:20] Simultaneous Neuronal and Blood Flow Imaging in Dorsal Striatum

[00:15:00] Conclusion

[00:16:33] Q&A

       _{PRESENTATION HIGHLIGHTS:}

• [00:00:10] Introduction to the main workhorse for multiphoton imaging: Ultima 2P^Plus
• [00:01:18] Introduction to UltraFast Volumetric Imaging.

[00:02:10] NeuraLeap module for increased volumetric imaging using a combination of methods:

• [00:02:30] Extending the Depth of Field.

• [00:04:42] Controlling Depth of Field with a DMD.
• [00:05:29] Using extended DOF as an ultrafast Z-device.
• [00:07:48] Using DMD selection for optical mixing.

[00:08:30] Summary

[00:09:39] Q&A