Topics discussed will include:
Coordinated activity across networks of neurons is a hallmark of both resting and active behavioral states in many species. These global patterns alter energy metabolism over seconds to hours, which underpins the widespread use of oxygen consumption and glucose uptake as proxies of neural activity. However, whether changes in neural activity are causally related to metabolic flux in intact circuits on the timescales associated with behavior is unclear.
Here, we combine two-photon microscopy of the fly brain with sensors that enable the simultaneous measurement of neural activity and metabolic flux, across both resting and active behavioral states. We demonstrate that:
As the relationship between neural activity and energy metabolism is probably evolutionarily ancient and highly conserved, our studies provide a critical foundation for using metabolic proxies to capture changes in neural activity.
Learn more about Bruker's multiphoton microscopes or our other solutions for the investigation of neural and metabolic networks:
SEE MORE OF THIS PRESENTER'S RESEARCH:
Kevin Mann, Ph.D., Applications Scientist, Bruker
Dr. Kevin Mann received Ph.D. degree from the University of California, Berkeley under the guidance of Dr. Kristin Scott where he studied fundamental behaviors in Drosophila using genetics, multiphoton microscopy, and electrophysiology. Next, he moved on to postdoctoral training in the laboratory of Dr. Tom Clandinin at Stanford University. Collaboratively he developed a method for whole-brain calcium imaging to detail the intrinsic functional neuronal network in Drosophila.