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Virtual Event, Actual Science

European BioAFM User Meeting [2022]

Learn about the latest advancements in BioAFM technology and the leading-edge research supported by our state-of-the-art systems
Now Available On Demand


Discover leading-edge BioAFM research and the latest advances in BioAFM instrumentation.

An impressive panel of researchers share insights into their work using Bruker BioAFM technology.

Featuring real-time demonstrations of state-of-the-art BioAFM instruments—including the newly launched NanoWizard V BioScience AFM—recorded LIVE from our laboratories in Berlin.

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Find out more about the technology featured in this event or our state-of-the-art BioAFM technology:

Agenda

Time (CET) Topic Speaker(s)
14:00 Welcome & Introduction Peter Dewolf, Ph.D., Worldwide Application Director, Bruker Nano Surfaces and Metrology
Thomas Henze, Ph.D., Head of Applications BioAFM, Berlin, Germany
14:05 Latest BioAFM Developments Thomas Henze, Ph.D., Head of Applications BioAFM, Berlin, Germany

Session 1: Mechanics, Large Samples, and Tissues

Time (CET) Topic Speaker(s) Abstract
14:20 Single Cell Compression Using AFM Katarzyna Pogoda, Ph.D., Institute of Nuclear Physics, Polish Academy of Sciences, Poland Read More
14:45 AFM and Fluorescence Microscopy on Human Osteoarthritic Cartilage: Correlation of Chondrocyte Organization, Nanomechanics and Collagen Structure Across Length Scales Mathäus Tschaikowsky, Institute of Physical Chemistry, University of Freiburg, Germany Read More
15:10 Live Lab Demo: NanoWizard V Tanja Neumann, Ph.D., Bruker BioAFM, Berlin, Germany  
15:30 Coffee Break    

Session 2: High-Resolution & Fast Imaging, Super-Resolution Microscopy

Time (CET) Topic Speaker(s) Abstract
15:35 Phase Separation in the Outer Membrane of Escherichia coli Georgina Benn, Ph.D., Silhavy lab, Princeton University, USA Read More
16:00 AFM-STED Correlative Microscopy Claudio Canale, Ph.D., Department of Physics, University of Genoa, Italy Read More
16:25 Live Lab Demo: NanoWizard ULTRA Speed 2 Andre Körnig, Ph.D., Bruker BioAFM, Berlin, Germany  
16:50 Q&A and Closing Peter Dewolf, Ph.D., Worldwide Application Director, Bruker Nano Surfaces and Metrology
Thomas Henze, Ph.D., Head of Applications BioAFM, Berlin, Germany		  

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Speakers and Abstracts

With introduction, Q&A, and conclusion led by Peter Dewolf, Ph.D., Worldwide Application Director, Bruker Nano Surfaces & Metrology, and Thomas Henze, Ph.D., Head of Applications BioAFM

Single Cell Compression using AFM

Katarzyna Pogoda, Ph.D., Institute of Nuclear Physics, Polish Academy of Sciences, Poland


ABSTRACT: During the talk, I will discuss how to perform uniaxial compression of single cells via Atomic Force Microscopy and how to obtain stress-strain curves to describe compression-stiffening behaviour in single cells.

ABOUT THE PRESENTER: Dr. Katarzyna Pogoda has an M.Sc in Biophysics and holds a Ph.D. in Physics. She was a Fulbright Fellow and a Postdoc at the University of Pennsylvania, USA. Currently, she is employed as an Assistant Professor at the Institute of Nuclear Physics PAN, Poland. Dr. Pogoda has been working with Atomic Force Microscopy since 2010. In her work, she has been exploring the rheological properties of live cells in the context of their cancerous transformation.

AFM and Fluorescence Microscopy on Human Osteoarthritic Cartilage: Correlation of Chondrocyte Organization, Nanomechanics and Collagen Structure across Length Scales

Mathäus Tschaikowsky, Institute of Physical Chemistry, Albert-Ludwigs-University of Freiburg, Germany


ABSTRACT: While it is well established that many tissues, such as cartilage, consist of hierarchical structures, we still barely understand how different structural length scales determine the properties of these tissues. One reason for this is that, currently, nanoscale information can only be obtained very locally and often cannot be related to larger scales. This limits our ability to gain insights into the understanding of diseases related to biomechanics, such as osteoarthritis. Here, we demonstrate how a hybrid fluorescence-AFM setup provides both mechanical and topographic information of osteoarthritic cartilage with nanoscale resolution over a range of several millimeters. 

ABOUT THE PRESENTER: Mathäus Tschaikowsky is a doctoral researcher and part of Dr. Bizan Balzer’s team in the lab of Prof. Dr. Thorsten Hugel at the Institute of Physical Chemistry in Freiburg. In cooperation with G.E.R.N. lab (University Medical Center Freiburg), he is studying the nanomechanical and morphological changes in human articular cartilage at the early stages of osteoarthritis. Using Atomic Force Microscopy in combination with fluorescence microscopy, he could study the pathology of the tissue at the nanometer scale.

Phase Separation in the Outer Membrane of Escherichia coli

Georgina Benn, Ph.D., Silhavy Lab, Princeton University, USA


ABSTRACT: The outer membrane protects Gram-negative bacteria against toxins and antibiotics, thereby contributing to antimicrobial resistance. However, relatively little is known about how the constituents of this vital membrane are organised. By combining labelling and genetics with atomic force microscopy we determine, at molecular resolution, the organisation of the outer membrane of living Escherichia coli. This allows us to conclude that the outer membrane is a mosaic of phase-separated lipopolysaccharide and protein regions, the maintenance of which is essential to the integrity of the membrane during growth and hence to the lifestyle of a Gram-negative bacterium.

ABOUT THE PRESENTER: After studying Biochemistry at King’s College London, Georgina became a PhD student in Bart Hoogenboom’s group at the London Centre for Nanotechnology, University College London, in 2017. Here, she used atomic force microscopy (AFM) to study the surfaces of living bacteria with nanometre resolution. She combined AFM with molecular biology to look at the native architecture of the outer membrane, as well as how proteins and peptides affect these membranes to kill bacteria. She is now a Postdoc in the Silhavy lab in Princeton University, having graduated from Bart’s group in December 2021. 

AFM-STED Correlative Microscopy

Claudio Canale, Ph.D., Department of Physics, University of Genoa, Italy

ABSTRACT: AFM-STED correlative microscopy was presented for the first time in 2012. In the last decade, this integrated technology has been employed to overcome some of the limits of single technique analysis. In this talk, the development of AFM-STED microscopy will be reviewed, starting from the motivation that drove the development of the first system to recent applications.

ABOUT THE PRESENTER: Dr. Claudio Canale, graduated in Physics, did his Ph.D. in Biophysics, and is currently a Senior Researcher at the University of Genoa, Italy. He began working on AFM in 2002 and has worked for many years on the study of the biological mechanisms associated with neurodegeneration in misfolding protein diseases. He contributed to the development of the first AFM-STED setup in 2012 while working at the Department of Biophysics of the Italian Institute of Technology.

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Explore the latest innovations in bioAFM technology.

Recordings include nearly 2.5 hours of exclusive content.

Viewers can expect to see:

  • Demonstrations of the NanoWizard V BioScience AFM and NanoWizard ULTRA Speed 2 AFM platforms;
  • Four expert-led lectures on the latest techniques in correlative microscopy and cell biology research; and
  • Detailed explorations of leading-edge BioAFM research, including experimental setup and data analysis processes.

 

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DNA-PAINT is a localization-based super-resolution method offering molecular resolution (< 5 nm) combined with unlimited multiplexing capabilities. DNA-PAINT reagents from Massive Photonics are a perfect match for the Bruker Vutara VXL microscope. In combination with the Bruker fluidic system, automated, multiplexed cellular imaging in 3D is now accessible out of the box.

Watch the webinar for practical guidance for DNA-PAINT sample preparation, image acquisition, and data analysis, as well as an introduction to:

  • The Vutara VXL super-resolution microscope and integrated fluidics unit;
  • The theoretical background of DNA-PAINT; and
  • The products available from Massive Photonics for DNA-PAINT experiments.

 

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