Light-Sheet Microscopes
For fast, 3D imaging of live or cleared-tissue samples
See Biology Across All Scales
With a strong focus on applications, our light-sheet solutions are easy to use and enable scientists around the globe to excel in their research. The SPIM technology avoids sample phototoxicity by sequentially illuminating a stack of thin slices of the organism, allowing scientists to observe living organisms for extended periods of time without photodamage.
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Browse Our Light-Sheet Microscopy Library
Browse Light-Sheet Microscopy Articles
| Year | Journal | Title | Author(s) | Imaging Platform | Subject | Methodology |
|---|---|---|---|---|---|---|
| 2024 | Cell Press | A mechanical wave travels along a genetic guide to drive the formation of an epithelial furrow during Drosophila gastrulation | A Popkova, U Andrenšek, S Pagnotta, P Ziherl, M Krajnc, M Rauzi | MuVi SPIM | morhphogenesis, epithelial furrowing, pacemakers | Drosophila, imaging, cytoskeleton |
| 2024 | Science | Temporal variability and cell mechanics control robustness in mammalian embryogenesis | D Fabrèges, B Corominas-Murtra, P Moghe, A Kickuth, (..) Takashi Hiiragi | InVi SPIM | Pre-implantaion development, embryogenesis, Temporal variability and cell mechanics, | mouse, rabbit and monkey; immunostaining, chemical inhibitors, in silico modelling, image analysis |
| 2024 | Communications Medicine | Structural reconstruction of mouse acute aortic dissection by intravenously administered human Muse cells without immunosuppression | M Takahashi, Y Kushida, Y Kuroda, S Wakao, Y Horibata, H Sugimoto, M Dezawa & Y Saiki | MuVi SPIM | Stanford type B acute aortic dissection (Stanford-B AAD), Muse cells | Muse cells, acute aortic dissection, Stanford type B, intravenous injection, sphingosine-1-phosphate, elastin, HLA-G |
| 2024 | RNA | Boosting the toolbox for live imaging of translation | M Bellec, R Chen, J Dhayni, (..) Jeremy Dufourt | MuVi SPIM | Live imaging of translation | drosophila, mammalian cells, nanobody/tag system, ALFA_array |
| 2024 | Ecotoxicology and Environmental Safety | Effects of water fluoridation on early embryonic development of zebrafish | Y-L Wei, X-C Lin, Y-Y Liu, Y-Q Lei, X-D Zhuang, H-T Zhang, X-R Wang | MuVi SPIM | fluoridation, environmental toxicology, embryogenesis, development | drug assay, LD50, zebrafish |
| 2024 | Nature Methods | Image restoration of degraded time-lapse microscopy data mediated by near-infrared imaging | N Gritti, R M. Power, A Graves & J Huisken | MuVi SPIM | time-lapse microscopy, image degradation, image restoration, AI, machine learning, neural network | AI, machine learning, neural network, near infra-red imaging |
| 2024 | Scientific Reports | Visualisation of gene expression within the context of tissues using an X-ray computed tomography-based multimodal approach | K Kairišs, N Sokolova, L Zilova, C Schlagheck, R Reinhardt, T Baumbach, T Faragó, T van de Kamp, J Wittbrodt & V Weinhardt | MuVi SPIM | multimodal imaging and registration, gene expression visualization, medaka | X-ray CT, light sheet microscopy, multimodal data |
| 2024 | JMRI | Assessment of Tumor Cell Invasion and Radiotherapy Response in Experimental Glioma by Magnetic Resonance Elastography | Fels-Palesandro et al. | LCS SPIM | glioma, MRI, tissue clearing | multimodal data, coregistration |
| 2024 | ResearchSquare preprint | Cross-species transcriptome analysis of the subthalamic and para-subthalamic nuclei reveal mRNA patterns unique to primate and relevant to neuropsychiatry | S Dumas, A Ricci, E Rubino, M Rosenkilde, C Francois, C Karachi, Å Wallen-Mackenzie | LCS SPIM | mRNA patterns, neuropsychiatry, brain regionalization | mRNA patterns, primate, mice, tissue clearing |
| 2024 | Front. Immunol. | Impaired meningeal lymphatic drainage in Listeria monocytogenes infection | J Feng, Y Ren, X Wang, X Li, X Zhu, B Zhang, Q Zhao, X Sun, X Tian, H Liu, F Dong, X-L Li, L Qi, and B Wei | MuVi SPIM | Listeria monocytogenes, bacterial infection, cellular polarization, inflammation, lymphangiogenesis | Mice, rtPCR, HE staining, tissue clearing, cytometry |
| 2024 | Development | Nuclear instance segmentation and tracking for preimplantation mouse embryos | Nunley et al. | |||
| 2024 | Thesis Harvard | Use of the Zebrafish to Explore the Connection of Pituitary Development with Congenital Craniofacial Syndromes | Tsay | craniofacial malformations, Esrp1/2, lhx3, embryogenesis | zebrafish, WISH, RNAscope, Crispr/Cas9, morpholino | |
| 2024 | Scientific Reports | Tumor biomechanics as a novel imaging biomarker to assess response to immunotherapy in a murine glioma model | Streibel et al. | LCS SPIM | MRI, MRE, glioblastoma, glioma | Multiparametric MRI and MRE, Tissue clearing and LSM, immunohistochemistry, image analysis |
| 2024 | biorxiv preprint | Creased ciliary flocks shape unfolding dynamics via information bottlenecks in an aneural animal | C Brannon and M Prakash | TruLiv3D Imager | Placozoa, Trichoplax adhaerens, epithelial folding, active matter, active solids, active origami, collective behavior, cilia, flocking, information bottlenecks | tracking, in situ observation, chemical inhibition, mathematical analysis |
| 2024 | Cancer Cell | Distinct tumor architectures and microenvironments for the initiation of breast cancer metastasis in the brain | Gan et al | MuVi SPIM | TNC spheroid, microglia, tumour response, perivascular, brain metastasis | cell lines, mouse, immunohistochemistry, Oncosphere culture, In vitro cell proliferation assay, In vitro wound scratch assay, CRISPRi |
| 2024 | Current Biology | Inflation-induced motility for long-distance vertical migration | A Larson, R Chajwa, H Li, M Prakash | InVi SPIM | cell buoyancy, dinoflagellate, marine ecology, cell size, aquaporins, osmotic regulation, biological pump, plankton motility, cell migration, Stokesian sedimentation | Vertical tracking experiments, Cell culture, Image processing, Electron microscopy, Protein structure prediction |
| 2024 | Nature Communications | A nanoparticle-based sonodynamic therapy reduces Helicobacter pylori infection in mouse without disrupting gut microbiota | T Liu, S Chai, M Li, X Chen, Y Xie, Z Zhao, J Xie, Y Yu, F Gao, F Zhu & L Yang | MuVi SPIM | antimicrobial resistance, nanoparticle, gastric infection, antibiotic resistance | Helicobacter pylori, mouse, 16 S rRNA gene sequencing for gut microbiota |
| 2024 | biorxiv preprint | Self-organization of vascularized skeletal muscle from bovine embryonic stem cells | M Sanaki-Matsumiya, C Villava, L Rappez, K Haase, J Wu, M Ebisuya | MuVi SPIM | bovine ESC, beef, PSM, muscle fibre, sarcomeres | Bovine ESC culture, Bovine muscle induction protocol in 2D/3D, Immunohistochemistry, Time-lapse, calcium oscillation |
| 2024 | biorxiv preprint | Adult oligodendrogenesis gates arcuate neuronal glucose sensing through remodelling of the blood-hypothalamus barrier via ADAMTS4 | Buller et al | LCS SPIM | hypoglycaemia, diabetes, ADAMTS4, oligodendrogenesis | mouse, Glucose challenges, Hyperinsulinaemic clamp paradigm, Metabolic tolerance tests,Tissue Processing, imaging, image processing |
| 2024 | biorxiv preprint | KIF2A maintains cytokinesis in mouse embryonic stem cells by stabilising intercellular bridge microtubules | Stockmann et al | InVi SPIM | cytokinesis, cell division, KIF2A | cell culture, antibodies, transgenic mESC lines, immunofluorescence, imaging, image analysis |
| 2023 | The EMBO Journal | Embryo-uterine interaction coordinates mouse embryogenesis during implantation | V Bondarenko, M Nikolaev, D Kromm,(..)Takashi Hiiragi | InVi SPIM | Pre-implantaion development, Morphogenesis, Patterning | Mouse, Ex-vivo, Live imaging, Laser ablation |
| 2023 | Journal of Neuroscience | A Cellular Ground Truth to Develop MRI Signatures in Glioma Models by Correlative Light Sheet Microscopy and Atlas-Based Coregistration | K Schregel, L Heinz, J Hunger, C Pan, (..) Michael O. Breckwoldt | LCS SPIM | glioblastoma, tumoral MRI biomarkers, co-registration, multi-modal | mouse, MRI, LSFM, coregistration, segmentation, image analysis |
| 2023 | biorxiv preprint | A novel ground truth dataset enables robust 3D nuclear instance segmentation in early mouse embryos | H Nunley, B Shao, P Grover, J Singh, B Joyce, (..) Lisa M. Brown | InVi SPIM | preimplantation, dataset BlastoSPIM | mouse, 3D instance segmentation, convolutional neural network, StarDist 3D |
| 2023 | Clinical and Translational Medicine | Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer | S Sivakumar, S Lieber, D Librizzi, (..) Sabine Müller-Brüsselbach, Rolf Müller | LCS SPIM | ovarian cancer, metastasis | Basal cell adhesion molecule (BCAM) is a laminin α5 (LAMA5), Biochemical, omics-based and real-time cell assays |
| 2023 | Advanced Science | The Role of PRRC2B in Cerebral Vascular Remodeling Under Acute Hypoxia in Mice | S Li, W Hu, S Gong, P Zhang, J Cheng,(..) Zengqiang Yuan | MuVi SPIM | hypoxia | mouse, high altitude exposure, cerebral vasculature, RNA immunoprcipitation-seq, and transcriptomic co-analysis |
| 2023 | Thesis University Heidelberg | Designing Deep Learning Frameworks for Plant Biology | L Cerrone | image processing | plants, PlantSeg, machine learning, data analysis | |
| 2023 | biorxiv preprint | Pre-patterned epithelial invagination prevents mechanical instability during fly gastrulation | BC Vellutini, MB Cuenca, (..) Pavel Tomančák | MuVi SPIM | cephalic furrow | Drosophila, epithelial fold, biomechanics, in vivo perturbations and in silico simulations |
| 2023 | biorxiv preprint | Distinct tumor architectures for metastatic colonization of the brain | S Gan, DG Macalinao, SH Shahoei, (..) Joan Massagué | MuVi SPIM | Brain metastasis, breast cancer | cell culture, mouse, patient tissue sections, single-cell transcriptomic, imunnostaining, gene overexpression and knowckdown |
| 2023 | Thesis Princeton | Maintaining Bilateral Symmetry and Terminal Development in Early Drosophila Embryogenesis | CM Smits | MuVi SPIM | embryogenesis, symmetry | drosophila |
| 2023 | Plant Biology | Root-specific photoreception directs early root development by HY5-regulated ROS balance | Li, Zeng, Tian, Zhao | MuVi SPIM | root development, plants, ROS balance | |
| 2023 | Theranostics | In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma | Hunger et al. | LCS SPIM | glioma, tumor microenvironment, iron oxide nanoparticles, immunotherapy, adoptive T cell therapy, non-invasive treatment monitoring | car T cell therapy, nanoparticles, MRI, tissue clearing |
| 2023 | biorxiv preprint | Tgfbr1 regulates lateral plate mesoderm and endoderm reorganization during the trunk to tail transition | A Lozovska, A Nóvoa, Y-Y Kuo, A D. Jurberg, G G. Martins, A-K Hadjantonakis, M Mallo | MuVi SPIM | Tgfbr1, lateral plate mesoderm (LPM), embryogenesis, development, EMT | mouse, WISH, ex vivo cell culture |
| 2023 | Thesis UNIVERSIDAD POLITÉCNICA DE MADRID | Marina Herrero Juanco | MuVi SPIM | Arabidopsis, lateral root development | lineage tracing, light sheet imaging | |
| 2023 | Current Biology | Maintaining symmetry during body axis elongation | C M. Smits, S Dutta, V Jain-Sharma, S J. Streichan, S Y. Shvartsman | MuVi SPIM | embryogenesis, symmetry | drosophila |
| 2023 | Thesis Universität Marburg | Role of BCAM in ovarian cancer metastasis | Suresh Sivakumar | LCS SPIM | ADAM10, BCAM, ovarian cancer, spheroids | Biochemical, omics-based, mouse, imaging |
| 2023 | Thesis University of Notre Dame | Mechanisms of Crosstalk and Signal Integration of Calcium Signaling in Epithelial Tissues | V Velagala | MuVi SPIM | organogenesis, development, epithelial tissue, biochemisty | Drosophila melanogaster, multiscale modelling, mathematical modelling, image processing |
| 2023 | Thesis University of Konstanz | Generation and functional characterization of specific astrocyte subpopulations | A-S Spreng | MuVi SPIM | astrocytes, nervous system, iPSCs, inflammatory cytokines, | organoids, iPSC-generated astrocytes, cytokine stimulation |
| 2023 | Thesis Weill Cornell Graduate School | Next Generation Cerebral Organoids: The Importance of Cell-Type Diversity in Modeling Proper Human Brain Development | C A Rittenhouse | TruLiv3D Imager | ||
| 2022 | Nature | Periodic formation of epithelial somites from human pluripotent stem cells | M Sanaki-Matsumiya, M Matsuda, N Gritti… Miki Ebisuya | MuVi SPIM | embryonic development, somitogenesis, segmentation clock | Human iPSC cultures, human somitoids, human somitoids, HCR, IHC, timelapses, human somitoids, morphometry |
| 2022 | National Acad Sciences | Dynamics of Drosophila endoderm specification | SE Keenan, M Avdeeva, L Yang… Stanislav Y. Shvartsman | MuVi SPIM | Drosophila 4D imaging, developmental patterning, gene regulatory network | drosophila, Time-lapse microscopy, single-cell, modelling, image analysis |
| 2022 | Mammary Stem Cells: Methods | Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction | LG Del Valle, MG Montero, M Jechlinger | InVi light-sheet | organoid culture optimization | mouse, organoid cultures, primary donor tissue, 3D acini |
| 2022 | Nature | Embryo-scale epithelial buckling forms a propagating furrow that initiates gastrulation | J Fierling, A John, B Delorme, A Torzynski… Matteo Rauzi | biomechanics, tissue buckling, mesoderm internalization | Drosophila , actomyosin contraction, computational models, in toto embryo image analysis and manipulation | |
| 2022 | Cell | An ex vivo system to study cellular dynamics underlying mouse peri-implantation development | Takafumi Ichikawa, Hui Ting Zhang, Laura Panavaite, ... Takashi Hiiragi | peri-implantation embryo development, mechano-chemical interactions | mouse, 3D ex vivo culture | |
| 2022 | biorxiv preprint | A topologically complex cytoplasm enables inflation-based escape from a gravity trap | AG Larson, R Chajwa, H Li, M Prakash | InVi light-sheet | biophysics, gravity | phytoplankton dinoflagellate Pyrocystis noctiluca, toroidal cytoplasm |
| 2022 | Thesis University Heidelberg | A topologically complex cytoplasm enables inflation-based escape from a gravity trap | AG Larson, R Chajwa, H Li, M Prakash | InVi SPIM | Pre-implantaion development, Morphogenesis, Patterning | Mouse, Ex-vivo, Live imaging, Laser ablation |
| 2022 | https://link.springer.com/protocol/10.1007/978-1-0716-2659-7_7 | no access | ||||
| 2022 | biorxiv preprint | Progressive maturation of the root apical meristem in Arabidopsis thaliana lateral roots | B Berthet, L Bald, M Louveaux, A Maizel | MuVi SPIM | lateral root development, stem cell niche | Arabidopsis thaliana, imaging, histochemistry |
| 2022 | The Lancet | Müller glia fused with adult stem cells undergo neural differentiation in human retinal models | SÀ Bonilla-Pons, S Nakagawa, EG Bahima… Maria Pia Cosma | MuVi SPIM | retina organoids, Müller glia, regeneration | organotypic cultures of human retina, preparations of dissociated cells, microinjection system, qRT-PCR, immunofluorescence, Flow cytometry |
| 2022 | Genome Biology | Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts | T De Coster, H Masset, O Tšuiko, M Catteeuw, Y Zhao… Joris Robert Vermeesch | InVi SPIM | genome segregation, zygotic division | Bovine embryo in vitro production, imaging, genome analysis, Nuclear staining, human biopsies |
| 2022 | iScience | Discovery of a novel SHIP1 agonist that promotes degradation of lipid-laden phagocytic cargo by microglia | C Pedicone, S Fernandes, A Matera, ST Meyer, S Loh…William G. Kerr | InVi SPIM | degradation of Synaptosomes, phagocytic degradation, microglia | artificial intelligence, mice, computational method, CRISPR/Cas9, immunofluorescence, flow cytometry |
| 2022 | STEM CELLS AND REGENERATION | Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids | D Medina-Cano, EK Corrigan, RA Glenn… Thomas Vierbuchen | TruLive3D Imager | developmental biology, organoids | mouse, pluripotent stem cells, Wnt pathway inhibitors, definitive endoderm and neural organoids,differentiation models |
| 2022 | Science | Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals | A Torres-Méndez, S Pop, S Bonnal, I Almudi, .. Manuel Irimia | MuVi SPIM | phylogeny, evolution, neuronal transcriptomic complexity | neuronal imaging experiments and cell type–specific rescues |
| 2022 | EMBO Reports | PLETHORA‐WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance | RC Burkart, VI Strotmann, GK Kirschner, A Akinci…Yvonne Stahl | MuVi SPIM | stem cell niche | Arabidopsis, lateral root primordium, cloning, staining, Nicotiana benthamiana infiltration, SCN staining, RNA staining, imaging, image analysis |
| 2022 | thesis Exeter | The Histone Methyltransferase KMT2D Suppresses SHH-Driven Medulloblastoma Tumor Progression and Metastasis | Sanghrajka, Reeti Mayur | LCS SPIM | malignant pediatric brain tumor, tumorigenesis | mouse, transcriptomic and chromatin profiling |
| 2022 | Developmental Cell | Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster | I Miguel-Escalada, MA Maestro, D Balboa, A Elek… Jorge Ferrer | MuVi SPIM | polygenic disease, pancreas agenesis, enhancer cluster | immunohistochemistry, RT-PCR, Flow cytometry, Seq analysis, ChIP-Seq analysis |
| 2022 | Cell Reports | The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions | CF Hevia, C Engel-Pizcueta, F Udina, C Pujades | MuVi SPIM | hindbrain morphogenesis, Notch signalling, stem cells, neurogenesis | zebrafish, CRISPR-Cas9, ISH, pharmacological treatments, immunohistochemistry, imaging, image analysis |
| 2021 | eLife | Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development | L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck… Joachim Wittbrodt | MuVi SPIM | morphogenesis and differentiation | medaka and zebrafish, embryonic pluripotent stem cells, fluorescent labeling, quantification |
| 2021 | Plant & Cell Physiology | Integration of Cell Growth and Asymmetric Division during Lateral Root Initiation in Arabidopsis thaliana | LM Schütz, M Louveaux, A Vilches Barro…Alexis Maizel | MuVi SPIM | Lateral root formation, periclinal division | Arabidopsis thaliana, four-dimensional reconstruction, lineage tracking, segmentation |
| 2021 | Biophysical Journal | Pinching and pushing: fold formation in the Drosophila dorsal epidermis | V Velagala, JJ Zartman | MuVi SPIM | planar epithelial sheets, morphogenesis, Hedgehog signalling | Drosophila, immunostaining, image processing, image analysis |
| 2021 | Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces | no access | ||||
| 2021 | BMC Dev Biol | The migratory pathways of the cells that form the endocardium, dorsal aortae, and head vasculature in the mouse embryo | C Collart, A Ciccarelli…J. C. Smith | MuVi SPIM | Vasculogenesis, embryogenesis | mouse embryo, single-cell transcriptomics, imaging, OPT |
| 2021 | Developmental Cell | A two-tier junctional mechanism drives simultaneous tissue folding and extension | A John, M Rauzi | MuVi SPIM | adherens junctions, tissue folding and extension, embryogenesis | Drosophila, In toto 4-D imaging, digital reconstruction, data processing, Drug injections, Dendra photoconversion, Cry2 optorecruitment, Actomyosin laser ablation |
| 2021 | Thesis Princeton | A Multiscale Characterization of ERK Signaling in the Early Drosophila Embryo | SE Keenan | MuVi SPIM | ||
| 2021 | ELife | Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach | A Seleit, A Aulehla, A Paix | TruLive 3D Imager | technology Crispr/Cas9, protocol | Crispr/Cas9, medaka, whole-genome sequencing |
| 2021 | Nature | Smad4 controls signaling robustness and morphogenesis by differentially contributing to the Nodal and BMP pathways | L Guglielmi, C Heliot, S Kumar, Y Alexandrov… Caroline S. Hill | MuVi SPIM | morphogens, morphometry | TGF-β family, SMAD4, zebrafish, Nodal, zebrafish, image analysis |
| 2021 | Thesis Princeton | Evolution-Guided Investigation of Developmental Mechanisms in Lungs of Terrestrial Vertebrates | MA Palmer | MuVi SPIM | PM | |
| 2021 | Thesis Heidelberg | Stem cell niche ontogeny during lateral root development in Arabidopsis thaliana | B Berthet | MuVi SPIM | ||
| 2021 | Cell Reports | Endothelial Wnts control mammary epithelial patterning via fibroblast signaling | J Wang, W Song, R Yang, C Li, T Wu, XB Dong, B Zhou… Yi Arial Zeng | MuVi SPIM | endothelial cells, Wnt, fibroblasts | fluorescent mouse, organoid, FACS, immnohistochemistry, RNA-seq |
| 2021 | Thesis Université Côte d’Azur, 2021. | Subcellular properties and embryo-scale dynamics driving morphogenesis | Alphy John | MuVi SPIM | ||
| 2020 | Semi-automatic generation of tight binary masks and non-convex isosurfaces for quantitative analysis of 3D biological samples | no access | ||||
| 2020 | Front. Plant Sci. | Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis | M Tichá, H Richter, M Ovečka, N Maghelli…Olga Šamajová | MuVi SPIM | Annexin 1, cell biology, lateral root growth | Arabidopsis, transgenic lines, immunoblotting, image analysis, Quantitative PCR |
| 2020 | Elife | Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation | A Alladin, L Chaible, L Garcia del Valle, R Sabine… Martin Jechlinger | InVi SPIM | cancer environment, oncogenes, breast cancer | mouse, 3D acinus cultures, qPCR analysis, image analysis, |
| 2020 | Cell Discovery | Light-sheet fluorescence imaging charts the gastrula origin of vascular endothelial cells in early zebrafish embryos | M Pang, L Bai, W Zong, X Wang, Y Bu, C Xiong… Jing-Wei Xiong | MuVi SPIM | cell lineage map, vascular endothelial cells | zebrafish, alignment, fusion, and extraction all-in-one software (AFEIO) for processing big data, quantitative analysis, photoconversion |
| 2020 | Macromolecular Biosciences | Poly(N‐isopropylacrylamide)‐Based Polymers as Additive for Rapid Generation of Spheroid via Hanging Drop Method | V RA, S Kumari, P Poddar, D Dhara… Souvik Maiti | MuVi SPIM | Multicellular tumor spheroid, protocol, tissue clearing | |
| 2020 | Journal of Biophotonics | Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution | P Matryba, A Wolny, M Pawłowska, ..Jakub Golab | tissue clearing | mouse penis | |
| 2020 | Elife | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy | C Prahst, P Ashrafzadeh, T Mead, A Figueiredo…Katie Bentley | MuVi SPIM | retina, opthalmology, vasculature | mouse, image processing |
| 2020 | Current Biology | Cell death in cells overlying lateral root primordia facilitates organ growth in Arabidopsis | S Escamez, D André, B Sztojka, B Bollhöner, H Hall… Hannele Tuominen | lateral root growth, cell death, cell biology | Arabidopsis, Gravitational induction of LRP initiation, Transmission electron microscopy, Light microscopy, Laser-assisted targeted cell elimination, RNA isolation and qPCR | |
| 2020 | Front. Bioeng. Biotechnol. | Opsef: Open source python framework for collaborative instance segmentation of bioimages | TM Rasse, R Hollandi, P Horvath | MuVi SPIM | Python framework for deep learning-based instance segmentation | Arabidopsis, image analysis |
| 2020 | Developmental Dynamics | Fusion of airways during avian lung development constitutes a novel mechanism for the formation of continuous lumena in multicellular epithelia | MA Palmer, CM Nelson | MuVi SPIM | avian lung development, epithelium | chicken, lung, photomanipulation |
| 2020 | Thesis Heidelberg | Studying the origins of primary tumours and residual disease in breast cancer | A Alladin | InVi SPIM | ||
| 2020 | ALTEX | Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies | M Brüll, AS Spreng, S Gutbier, D Loser, A Krebs… | MuVi SPIM | neuro-glial interactions , astrocytes, organoids | cell culture, proliferation, imaging |
| 2020 | Elife | Accurate and versatile 3D segmentation of plant tissues at cellular resolution | A Wolny, L Cerrone, A Vijayan, R Tofanelli, AV Barro… | MuVi SPIM | PlantSeg, cell segmentation | image analysis |
| 2020 | Current Biology | Axis specification in zebrafish is robust to cell mixing and reveals a regulation of pattern formation by morphogenesis | T Fulton, V Trivedi, A Attardi, K Anlas, C Dingare… | MuVi SPIM | morphogenesis, axis formation, development | zebrafish, explants,Mosaic Labeling, pharmacological inh, HCR, immunohistochemistry |
| 2020 | Developmental Cell | Tissue-scale mechanical coupling reduces morphogenetic noise to ensure precision during epithelial folding | AS Eritano, CL Bromley, AB Albero, L Schütz, FL Wen… | MuVi SPIM | development, epithelium, transcription | Drosophila, laser ablation, optogenetics, modelling |
System Comparison
* COMPATIBLE MODULE
| MuVi SPIM Multiview |
InVi SPIM Lattice Pro Inverted View |
LCS SPIM Large Cleared Samples |
TruLive3D Imager |
|
|---|---|---|---|---|
| Benchtop Design |
✓ | ✓ | ✓ | ✓ |
| Configuration |
Horizontal Multiple-View |
Inverted |
Inverted - Dual Illumination |
Inverted - Dual Illumination |
| # Lenses |
4 lenses (2 IO, 2 DO) |
2 lenses (1 IO, 1 DO) | 3 lenses (2 IO, 1 DO) | |
| Multi-View |
✓ | |||
| Live/Fixed Samples |
✓ | ✓ | ✓ | |
| Cleared Samples |
✓ | ✓ | ||
| Best Embedding |
Capillary/FEP tube w/ agarose 3D stage |
FEP foil; glass slides |
Quartz-crystal cuvette | TruLive3D dishes and FEP foil |
| Photomanipulation* |
✓ | ✓ | ✓ | |
| Environmental Control* | ✓ | ✓ | ✓ | |
| Destriping/Uniform Illumination* | ✓ | ✓ (w/ Advanced Illumination Module) | ✓ | ✓ |