On-Demand Program | 2 Hrs 20 Minutes

On Demand Event: European BioAFM User Meeting [2022]

Learn about the latest advancements in BioAFM technology and the leading-edge research supported by our state-of-the-art systems

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Welcome and Introduction

Presented by Peter Dewolf, Ph.D., Worldwide Application Director, Bruker Nano Surfaces & Metrology, and Thomas Henze, Ph.D., Head of Applications BioAFM (February 9, 2022)

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On-Demand Session | 15 Minutes

Latest BioAFM Developments

Presented by Thomas Henze, Ph.D., Head of Applications BioAFM, Berlin, Germany (February 9, 2022)

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[00:00:49] Overview - Features of the NanoWizard V BioScience AFM
[00:03:57] Highlight - NEW PeakForce-QI mode
[00:07:46] Highlight - NEW Fast-Scanning option
[00:13:13] Highlight - Contact resonance module

Summary:

In this presentation, Dr. Thomas Henze (Head of BioAFM Applications, Bruker GmbH) provides a comprehensive introduction to the NanoWizard V BioScience AFM and several other newly-available leading-edge operational modes, accessory modules, and software options.

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NanoWizard V BioScience

Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

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Contact Resonance Module

Enables quantitative characterization of nanomechanical properties up to 300 GPa based on contact resonance imaging

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On-Demand Session | 25 Minutes

Single Cell Compression Using AFM

Presented by Katarzyna Pogoda, Ph.D., Institute of Nuclear Physics, Polish Academy of Sciences, Poland (February 9, 2022)

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[00:01:08] Solid stress in brain tumors
[00:04:35] Brain tissue under compressive force
[00:07:01] Compression-stiffening in biological tissues
[00:11:09] Uniaxial compression via AFM
[00:14:04] Compression-stiffening of cells
[00:15:28] Large compression examples
[00:21:04] Mechanical response to compressive force
[00:25:00] Conclusion

Abstract:

During the talk, Katarzyna Pogoda, Ph.D (Institute of Nuclear Physics, Polish Academy of Sciences) discusses how to perform uniaxial compression of single cells via Atomic Force Microscopy and how to obtain stress-strain curves to describe compression-stiffening behaviour in single cells.

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Katarzyna Pogoda, et.al., Inhomogeneity of stiffness and density of the extracellular matrix within the leukoplakia of human oral mucosa as potential physicochemical factors leading to carcinogenesis, Translational Oncology, Volume 14, Issue 7, 2021, 101105, ISSN 1936-5233, https://doi.org/10.1016/j.tranon.2021.101105.

Cell Mechanics and Adhesion

Directly measure and quantify cell-substrate and cell-cell interactions at the single-cell level

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On-Demand Session | 20 Minutes

Live Lab Demo - NanoWizard V BioScience AFM

Presented by Tanja Neumann, Ph.D., Bruker BioAFM, Berlin, Germany (February 9, 2022)

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[00:00:30] System introduction
[00:03:37] Setting up an experiment
[00:07:17] Selecting a scan region
[00:09:30] Acquiring data

Summary:

In this presentation, Dr. Tanja Neumann (BioAFM Application Scientist, Bruker GmbH) demonstrates the new features and advanced capabilities of the NanoWizard V BioScience AFM.

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NanoWizard V BioScience

Automated quantitative nano-mechanical imaging for long-term, self-regulating experiment series.

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BioAFMs for Life Science

Providing unique insights into life sciences research including physical structure, biochemical interactions, and mechanical properties

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On-Demand Session | 20 Minutes

Phase Separation in the Outer Membrane of Escherichia Coli

Presented by Georgina Benn, Ph.D., Silhavy lab, Princeton University, USA (February 9, 2022)

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[00:00:28] What is e. coli?
[00:03:55] Experimental setup
[00:05:30] Generating and examining nanometre maps of the bateria surface
[00:08:27] Process and outcomes of chemically labeling OmpF with CoIN-mCherry
[00:11:30] Identifying phase separated LPS
[00:14:22] Mislocalizing phospholipids in the cell membrane
[00:17:25] Conclusion & Q/A

Abstract:

The outer membrane protects Gram-negative bacteria against toxins and antibiotics, thereby contributing to antimicrobial resistance. However, relatively little is known about how the constituents of this vital membrane are organised. By combining labelling and genetics with atomic force microscopy we determine, at molecular resolution, the organisation of the outer membrane of living Escherichia coli. This allows us to conclude that the outer membrane is a mosaic of phase-separated lipopolysaccharide and protein regions, the maintenance of which is essential to the integrity of the membrane during growth and hence to the lifestyle of a Gram-negative bacterium.

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Benn G, et al. Phase separation in the outer membrane of Escherichia coli. Proc Natl Acad Sci U S A. 2021 Nov 2;118(44):e2112237118. doi: 10.1073/pnas.2112237118

bioafm-life-sciences-applications-teaser-BRUKER

Life Science Research

Obtain nanoscale structural details and mechanical properties of biomolecules and cells beyond the reach of traditional AFM

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On-Demand Session | 25 Minutes

AFM-STED Correlative Microscopy

Presented by Claudio Canale, Ph.D., Department of Physics, University of Genoa, Italy (February 9, 2022)

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[00:00:35] Coupling AFM with optical microscopy
[00:03:15] Super-resolution by STEM microscopy
[00:06:01] STED-AFM vs confocal-AFM
[00:12:11] Investigation of amyloid fibril formation
[00:19:40] Results
[00:23:06] Conclusion & Q/A

Abstract:

AFM-STED correlative microscopy was presented for the first time in 2012. In the last decade, this integrated technology has been employed to overcome some of the limits of single technique analysis. In this talk, the development of AFM-STED microscopy will be reviewed, starting from the motivation that drove the development of the first system to recent applications.

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Cosentino M, Canale C, Bianchini P, Diaspro A. AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging. Sci Adv. 2019 Jun 19;5(6):eaav8062. doi: 10.1126/sciadv.aav8062

Correlative AFM and STED Microscopy

Get complementary data from these two techniques to gain deeper insight into the specimen under investigation

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On-Demand Session | 25 Minutes

Live Lab Demo - NanoWizard ULTRA Speed 2

Presented by Andre Körnig, Ph.D., Bruker BioAFM, Berlin, Germany (February 9, 2022)

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[00:00:37] Experiment introduction
[00:03:26] Experiment setup
[00:05:00] Acquiring data
[00:19:17] Conclusion & Q/A

Summary:

In this presentation, Dr. Andre Körnig (BioAFM Application Scientist, Bruker GmbH) demonstrates the use of the NanoWizard ULTRA Speed AFM for high speed imaging. QI Mode is also demonstrated.

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NanoWizard ULTRA Speed 2

High-speed imaging and super-resolution AFM on inverted microscopes, paired with unparalleled flexibility.

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BioAFMs for Life Science

Providing unique insights into life sciences research including physical structure, biochemical interactions, and mechanical properties

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Q&A and Closing Remarks

Presented by Peter Dewolf, Ph.D., Worldwide Application Director, Bruker Nano Surfaces & Metrology, and Thomas Henze, Ph.D., Head of Applications BioAFM (February 9, 2022)

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