NanoIR Spectroscopy & Imaging: Recent Developments and Applications

_{PRESENTATION HIGHLIGHTS}

• [00:00:47] Review of Abbe's diffaction limit, photothermal AFM-IR, and PeakForce Tapping
• [00:09:05] Introduction to peak force infrared microscopy (PFIR)
• [00:14:25] PFIR for hard/rough samples
• [00:17:17] PFIR for soft samples
• [00:18:43] PFIR for aerosols
• [00:21:00] Other applications of PFIR
• [00:22:32] Specialized PFIR: multi-pulse PFIR, PFIR with pump/probe geometry
• [00:25:20] Specialized PFIR: Liquid-phase PFIR
• [00:31:48] Summary of PFIR capabilities

_{PRESENTATION HIGHLIGHTS}

• [00:01:06] Why use both raman and IR spectroscopy for structural characterization?
• [00:03:12] Why IR reveals a variety of spectra unavailable via raman spectroscopy 
• [00:05:34] How nano-raman and nanoIR overcome traditional spatial resolution limits
• [00:07:56] Demonstrating the complementarity of TERS and AFM-IR on viruses
• [00:12:30] Observing amino acid composition & secondary structure of viral particles
• [00:13:14] Structural analysis of oligomers as the fundamental cause of Parkinson's disease
• [00:16:56] Understanding how the secondary structural organization and surface structure information of populations of oligomers change

_{PRESENTATION HIGHLIGHTS}

• [00:00:20] Why surface sensitivity matters
• [00:01:43] Volume probing vs.surface probing
• [00:03:15] PMMA wedge demonstration sample
• [00:05:00] Demo: Resonance-enhanced mode
• [00:06:00] Demo: Surface sensitive mode setup and data acquisition
• [00:10:30] Demo: Finding the position at which the infrared signal saturates
• [00:11:05] Demo: Generating and interpreting the height image
• [00:13:12] Demo: Analyzing a new dataset

_{PRESENTATION HIGHLIGHTS}

• [00:00:48] Live setup and real-time scan with Bruker's new AFM-IR platform and Nanoscope 6 controller
• [00:03:55] Demo: Switching the laser wavenumber and interpreting signal inversion
• [00:05:00] Demo: Switching from sample scanning to tip scanning information
• [00:06:10] Demo: PeakForce QNM in air using infrared imaging instrumentation/configuration
• [00:09:57] Demo: Achieving unmatched lateral resolution in a PS-b-PMMA aggregate sample