Introduction to Solids LT-MAS DNP

Info:

This 4-day course is designed to give users an introduction to dynamic nuclear polarization (DNP) techniques and equipment. Users will gain hands-on experience operating low-temperature (LT) MAS probes as well as Bruker microwave sources (i.e. gyrotron & klystron). Additional topics include DNP sample preparation methods as well as tips for optimizing DNP performance. Upon completion of this course, users will be able to independently operate a Bruker DNP system.

Topics:

•  Introduction to DNP probes
  - LT-MAS and microwave component review
  - LT sample insertion and ejection
  - Rotor spin-up and spin-down procedures
  - Venturi probe operation
  - Changing the RF configuration

• Operation of LT-MAS cabinet
  - Cool-down and warm-up procedures
  - Parameter control and monitoring via Topspin
  - Cryogen handling

• DNP sample preparation
  - Polarizing and glassing agents
  - Preparation methods & rotor packing
  - Techniques for sample optimization
  - Biological vs. material sample preparation

• Gyrotron and klystron operation
  - Basic operation and maintenance
  - Microwave control parameters
  - Safety interlocks
  - Cross-polarized (CP) MAS DNP on standard samples

• NMR Field sweeping for DNP optimization
  - Measurement of DNP enhancements
  - Practices for safe field sweeping

Prerequisite:

This course requires graduate-level understanding of (and preferably hands-on experience with) MAS NMR techniques, including CP MAS experiments. For example, attendees should already be familiar with content of Bruker Solids Course (offered the preceding week).

Equipment:

1. Klystron-based 400 MHz LT-MAS DNP system.
2. Gyrotron-based 600 MHz LT-MAS DNP system.
3. 3.2, 1.9 and/or 1.3 mm LT-MAS DNP probes.

All attendees will be trained on both gyrotron and klystron systems

Instructors: one PhD Applications Scientist per instrument.

Date, Location:

March 10 - 13 2025, Billerica MA (open)
September 15 - 18 2025, Billerica MA (soon)

Location:

More Info: