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Knowledge Pack: Super-Resolution Microscopy

Get instant access to our most popular resources exploring super-resolution microscopy all in one place.

This knowledge pack includes:

- 1 full-length ebook

- 3 full-length protocols

- 1 full-length webinar recording

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[eBook] Super-Resolution Microscopy

^{RESOURCE TYPE: eBook [PDF]

LENGTH: 38 pages

DESCRIPTION:}

Readers can expect to learn more about:

The principles and advantages of super-resolution microscopy for life sciences research;
Super-resolution microscopy applications across life sciences disciplines (e.g., neuroscience, cell biology, genomics, and virology);
The common approaches to achieving beyond diffraction-limited imaging, including localization and labeling techniques;
Recent and cutting-edge super-resolution microscopy-related advancements (e.g., biplane technology, top-hat illumination, and microfluidics); and
Future trends in super-resolution microscopy research.

Protocol for Preparation of dSTORM Imaging Buffer

^{RESOURCE TYPE: Protocol [PDF]

LENGTH: 1 page

DESCRIPTION:}

This protocol can be used to prepare imaging buffer for super-resolution dSTORM imaging applications.

Cytoskeleton-Tubulin Fixation

^{RESOURCE TYPE: Protocol [PDF]

LENGTH: 1 page

DESCRIPTION:}

This protocol can be used to fix cytoskeleton-tubulin in cells for super-resolution microscopy applications.

Sample Preparation for Cell Culture Labeling

^{RESOURCE TYPE: Protocol [PDF]

LENGTH: 1 pages

DESCRIPTION:}

This protocol can be used to prepare cell culture samples for super-resolution microscopy applications.

Dye Selection and Labeling Strategies for Super-Resolution Microscopy

^{RESOURCE TYPE: Webinar Recording

LENGTH: 63 minutes

DESCRIPTION:}

During this webinar, discover basic requirements, practical considerations, and innovative tips for obtaining high-quality single-molecule localization microscopy data while imaging fixed cells.

Viewers will learn more about:

Basic principles of SMLM and how they relate to the selection of organic fluorophores;
Different labeling strategies and approaches to optimize fixation conditions based on structural targets; and
How different buffer compositions can impact dye performance during SMLM.