Carbaplex®
Real-time PCR test
Carbaplex® IVD PCR
The Carbaplex® IVD-CE PCR Kit is a multiplex real-time PCR test for the five most prevalent carbapenemase gene families; providing rapid results with high sensitivity and specificity without the need for culture.
Carbapenemase-producing Enterobacteriaceae (CPE)
Carbapenemase-producing Enterobacteriaceae (CPE) are a global public health risk. The genes coding for carbapenemases are readily transmissible in healthcare environments and cause outbreaks. Combatting the spread of these genes requires rapid identification of carriers, robust infection control and prevention procedures. Bruker’s Carbaplex® IVD PCR Kit enables the rapid identification of CPE colonized patients; allowing the efficient use of healthcare resources and appropriate patient management.
Clinical problem
CPEs are a serious healthcare problem. They are resistant to the carbapenem class of antibiotics, which are often considered the ‘last-line’ of therapy for treating multi-drug resistant gram-negative bacteria:
- Carbapenemase genes are readily transmissible from colonized patients, leading to outbreaks in healthcare settings.
- Carbapenem resistance greatly restricts treatment options for patients infected with CPE, and is associated with longer hospital stays, higher medical costs and increased mortality.
- CPE outbreaks are expensive.
- European Centre for Disease Prevention and Control (ECDC) recommends rectal screening with presumptive isolation for patients who are considered at high risk of carrying a CPE.
- Fast diagnostic turnaround time and communication to the infection control team is a key component of controlling the spread of CPE.
Molecular identification of five carbapenemase gene families
The Carbaplex® IVD PCR Kit is a multiplex real-time PCR assay for rapidly identifying patients carrying CPEs, detecting and differentiating the five most prevalent carbapenemase genes from a single sample:
- OXA-48-like (including OXA-181)
- KPC
- NDM
- VIM
- IMP
For the confirmation of carbapenemase activity, the MBT STAR®-Carba assay is available, which runs on the MALDI Biotyper® IVD systems.
Fast diagnostic turnaround
The Carbaplex® IVD PCR Kit provides results in under 3 hours, compared with days for culture-based screening approaches, helping the laboratory to rapidly inform the infection control team when time matters.
Compatibility
The Carbaplex® IVD PCR Kit is an easy-to-use master-mix format and is designed to be compatible with existing laboratory equipment, minimizing the need for dedicated bench space or additional training:
- ABI 7500
- ABI QuantStudio 5
- Bio-Rad CFX
- Hain FluoroCycler® XT
- Mic qPCR Cycler
- Qiagen RotorGene
Routine samples
The Carbaplex® IVD PCR Kit is suitable for use in high-throughput testing scenarios using DNA extracted from rectal swabs, or for confirmation testing of suspected isolates.
Please contact your local representative for availability in your country.
Not for sale in the USA.
More information
- Carbaplex® (Brochure), IVD-CE
- A. Otter et al. Clin. Microbiol. Infect. 2017, 23 (3), 188-196. Counting the cost of an outbreak of carbapenemase-producing Enterobacteriaceae: an economic evaluation from a hospital perspective, DOI:
- European Centre for Disease Prevention and Control. Evidence brief: Update on the spread of carbapenemase-producing Enterobacteriaceae in Europe – Summary of the May 2015 expert assessment. Stockholm: ECDC; 2015.
