Fundamental Developments in Infrared Spectroscopic Imaging for Biomedical Applications

Abstract

Infrared chemical imaging is a rapidly emerging field with new advances in instrumentation, data acquisition and data analysis. Relying on purely biochemical signatures rather than contrast from exogenous dyes and stains, infrared chemical imaging has the potential to revolutionize histopathology for improved disease diagnosis. In this review we discuss the recent advances in infrared spectroscopic imaging specifically related to spectral histopathology (SHP) and consider the current state of the field. Finally we consider the practical application of SHP for disease diagnosis and consider potential barriers to clinical translation highlighting current directions and the future outlook.