Webinar Recap: Imaging Hippocampal Microcircuitry Using Glass Microperiscopes
This webinar recap summarizes the presentation given by Dr. Michael Goard, associate professor at U.C. Santa Barbara, about his lab's use of microprisms, microperiscopes, and multiphoton microscopy to study neuronal microcircuitry in areas of the hippocampus involved in memory formation.
In our "Imaging Hippocampal Microcircuitry Using Glass Microperiscopes" webinar, Dr. Goard described a new approach for imaging neural activity in the transverse hippocampus using two-photon microscopy. Topics discussed include how his team used this approach to measure dendritic morphology and functional activity during behavior, the technology involved, and its advantages and disadvantages for functional imaging.
Dr. Goard's presentation covered his lab's methods and setup, their procedure, imaging results, and the general considerations for the use of glass microprisms for deep brain imaging.
Audience Q&A
Download the webinar recap or watch the recording to learn more about these and other topics.
- What is the setup of your objective?
- Can you tell us more about the interface at the objective?
- Is there a cover slip and is it attached to the prism?
- Did you try to Image with a 1-photon microscope?
- What are the advantages and disadvantages of using a miniscope over a stationary microscope?
- How big of a volume do you collect and at what temporal resolution?
- Did you assess if the prism lenses/periscopes possess any other notable optical aberrations (e.g. spherical aberrations)?
- In the spontaneous activity, observed in the hippocampus, do you notice any structure to it? Does this structure change with the experience that the animal has (stimulus or behavioral-driven)?