Twenty-First Century Clinical Proteomics

This webcast will showcase how various NIH-funded consortia aimed at the ability to analyse thousands of plasma samples to better understand the impact of infection, vaccination and development on the immune system. These projects accelerated the development of a high-throughput plasma proteomics pipeline that can handle thousands of samples while concomitantly mapping more than a thousand plasma proteins at throughputs of 100 samples per day.

The pipeline, with reagent costs in the dollar-per-sample range, has been tested and validated on more than 10,000 samples from cohorts in various disease and development contexts, showing exquisite robustness. Interestingly, the concepts of this pipeline are widely applicable to a range of many other body fluids even in a sample-sparing environment such as samples from premature newborns.

Key Learning Objectives:

• How proteomics can match genomics data to identify complex biomarker signatures in large cohorts
• Current challenges and opportunities for scalable and affordable plasma proteomics solutions
• Why robustness matters for large-scale proteomics studies and the latest MS technologies