NMR Application Webinars during Pandemic Situation

Duration: 

57 min 41 s

Aim:

Provide brief discussion of NMR Applications on Foods and Medicinal Plants.

Short Description:

Four topics are discussed

1. Introduction to NMR Screening – Herbs, Spices, Foods and Food Products
2. Food quality assessment, focusing on carbohydrates, fats, and vitamins in processed food and juices
3. Profiling and detecting adulteration/fraud in honey
4. Plant metabolomics: targeted and non-targeted

Target Audience:

For Scientists work in Food and Nutraceutical Quality Control field and researchers working in fingerprinting of medicinal plants.

Duration: 

57 min 35s 

Aim: 

Provide brief introduction to Quantitative NMR and factors that contributes to high accuracy and precision in QNMR data including good sample preparation. 

Short Description: 

For topics are disscussed

1. Introduction to Quantitative NMR
2. Good Sample Preparation
3. Data Analysis QNMR – TopSpin Eretic 2
4. Further resources: On-line NMR Courses 

Target Audience: 

Researchers from variety fields who want to explore on Quantitative NMR approach. 

Duration: 

1 hour 4 min 

Aim: 

This talk is designed to introduce users to the Topspin software. Topic covered includes common processing steps of 1D 1H Spectra. 

Short Description: 

TopSpin was designed with a highly intuitive interface utilizing the most widespread standards familiar from word processing, graphics or presentation programs, providing the same look-and-feel for your NMR applications on all supported platforms. In order to provide students, researchers and teachers with unlimited access to the best tools for off-line NMR processing, TopSpin4 Processing software is now made freely available to academic community and governmental institutions. Thus, it makes this premier NMR software become a great teaching tool for academia. 

Topspin software installation and configuration for Windows operating system are disscussed in Step-by-step PDF. 

For those who want to know more about TopSpin 1D, 2D processing, please watch the On-Demand link Basic NMR Webinars, Course 3. 

Target Audience: 

New comers and undergraduates who want to explore on TopSpin4 processing and customers who are working with TopSpin. 

Aim: 

To provide a short update on our customers and solutions in front line response to COVID-19 NMR research. 

Short Description: 

A short update on COVID-19 NMR project and how NMR can applied to addressed the secondary structure of SARS-CoV-2 RNA, and determined the protein stuctures including non-structural proteins and nucleocapsid of SARS-CoV-2 virus. An update on molecular phenotyping of SARS-CoV2 patients using NMR are disscussed. The initial result indicated that there is a high promise of using this approach for developing novel SARS-CoV 2 diagnostic test based on concept called Phenoconversion. 

Target Audience: 

Researchers from clinical and biomedical fields.

Aim: 

Provide brief introduction of NMR on Metabolomics Application and discussion on how to have a good start. 

Short Description: 

Applications of Metabolomics approach are very diverse, namely Natural Products Characterization, Prediction of phytomedicine efficacy and in vivo pharmacokinetic study, Quality control of herbal medicines and material authenticity, drug treatment in clinical study (pharmacometabolomics), impact of nutrition on health (foodomics) and many more. The reasons on why NMR is commonly used for metabolomics analysis are discussed. Appropriate objective/experimental design in Metabolomics research is utmost important and could be the determining factor on the success of the study. Factors that influences on chemical shifts are also discussed. 

Target Audience: 

Researchers from variety fields who want to explore on NMR-based Metabolomics approach. 

Aim: 

Provide brief introduction of NMR spectrometer. 

Short Description: 

Brief tour to new corporate Bruker Website 2021 and how to get the Free TopSpin Academia License. Introduction to NMR Spectrometer namely Benchtop and High Field instruments. Key components of NMR Spectrometer including Magnets, Electronic Console, Probes are discussed. The importance of Lock and Shimming as mandatory steps in NMR acquisition are explained. 

Target Audience: 

For newcomers to NMR Spectroscopy and undergraduates 

Metabolic phenotyping using nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS) has been applied to the characterization of the metabolic effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on human blood plasma. The hybrid NMR-MS model demonstrated a sensitivity of 100 % for SARS-CoV-2 infection, showing it to be an exceptionally powerful tool for the metabolic discrimination of samples infected by the SARS-CoV-2 virus. The signature of SARS-CoV-2 positivity includes elements of liver dysfunction, dyslipidaemia, diabetes, and coronary heart disease risk. Heating of blood samples to deactivate SARS-CoV-2 virus can lead to incorrect biomarker identification. Heat-treatment (56 °C for 30 minutes; the standard protocol used for inactivation of SARS CoV-2 in blood samples analyzed in laboratories without high-level biosecurity facilities) shown to degrade lipoproteins and differentially alter metabolic information. Data related to SARS CoV-2 obtained from heat-treated samples are largely uninterpretable and are of limited research value. 

Target audience:

Researchers who are working on clinical field, typically COVID-19 disease.

Good selection of NMR experiment could ensure optimize results which in turn deliver the maximum information of the mixture. 

Topics:

• NMR Experiments Background
• 1D and/or nD Experiments?
• Modern NMR experiments for Metabolomics
• Automatic Data Analysis – Enabling Tools 

Target audience:

Researchers who plan to collect good NMR data at the highest efficiency.

NMR Spectroscopy play a vital role in development of herbal medicines. “Classical” medicinal plant research required several fractionation steps until the isolation of pure chemical constituents. NMR spectroscopy is then applied to identify and characterize the pure natural products. As the technologies developed, the trends in herbal medicines research have changed. Nowadays, analyzing mixture sample from herbal medicines become routine especially the integration of chemometrics approach to NMR spectroscopy, or also known as Metabolomics. Therefore, NMR-based metabolomics could be applied to authenticate the nutraceutical products and medicinal materials. Particularly, in addition to taxonomic/variety information related to the herbal species, this method is powerful to discriminate the geographical and seasonal differences of herbal materials. Hence, making quality control of phytomedicines possible when non-targeted analysis (Multivariate statistical analysis) is applied on NMR data. 

Target audience:

Researchers from National center of natural products, Department of Pharmacognocy, Center of Phytochemistry, Natural Products Laboratory, Institue of Drug and Food Control 

Topics: Introduction to NMR spectroscopy 

• NMR Spectrometer: Spectrometer Console, Ascend Magnet, Probes, Automation, TopSpin, Fourier 80 (Benchtop NMR) 
• NMR Observables: Chemicifts, Integration and Multiplets 

Target audience:

Layman and undergraduates

Topic: 

NMR Application Overview

Target audience:

Layman and graduates