AFM in Conjunction with FluidFM® Technique and Applications

       _{PRESENTATION HIGHLIGHTS}

• [00:00:24] The role and mechanisms of chemical flocculation in harvesting microalgae
• [00:04:17] Known, hypothesized, and validated behavior and mechanisms of chitosan-induced flocculation 
• [00:08:48] Advantages and process of inducing flotation without flocculation using AFM and FluidFM technologies
• [00:15:38] Methods for probing the interaction between bubbles produced by FluidFM and living cells; how to functionalize these bubbles
• [00:19:59] Conclusions
• [00:21:33] Live Q&A with the presenter

       _{PRESENTATION HIGHLIGHTS}

• [00:00:13] Introduction to FluidFM technology
• [00:02:09] CS1: Repeated cytoplasma extraction and RNA sequencing from same cell
• [00:04:41] CS2: Studying virus-host cell interactions with colloid aspiration
• [00:06:46] CS3: Guided cell growth
• [00:07:42] CS4: Local treatment of cells
• [00:11:36] CS5: Inject mammalian cells in vitro
• [00:12:21] CS6: Inject cells w/ CRISPR reagents
• [00:15:09] 2021 FluidFM publications

       _{PRESENTATION HIGHLIGHTS}

• [00:00:18] Overview of NanoWizard family AFMs
• [00:02:15] Unique capabilities and advantages of the NanoWizard 4 XP
• [00:04:08] Integrating FluidFM into BioAFM
• [00:05:49] FluidFM for cell adhesion studies
• [00:06:12] FluidFM Cell Injection and Extraction applications
• [00:06:54] FluidFM Colloidal Force Spectroscopy mode applications
• [00:08:10] FluidFM Spotting applications
• [00:09:05] Combining FluidFM Spotting and AFM, including imaging with nanopipette

       _{PRESENTATION HIGHLIGHTS}

• [00:00:09] BioAFM-FluidFM system assembly and experiemnt overview
• [00:02:15] Configuring the software and collecting a cell
• [00:05:11] Performing the adhesion experiment
• [00:06:51] Reconfiguring the software to increase the probability that the cell will adhere to the substrate
• [00:07:45] Generating custom, complex, and combined force curves
• [00:09:43] Modeling the standard workflow for FluidFM-BioAFM experiments