Live Demos from Bruker's Mass Spec Lab

With the timsTOF Pro, powered by the PASEF^® acquisition modes, Bruker has largely contributed to take proteomics analysis to a new dimension, by removing the need to compromise speed for sensitivity or selectivity, while reaching unprecedented levels of robustness. Introduced @ ASMS 2024, the timsTOF Ultra 2 is the pinnacle of timsTOF evolution, combining ultimate sensitivity, speed, selectivity and dynamic range with the field-proven legendary timsTOF robustness. During this live from the lab, you will witness through real-life examples how sensitive and versatile the timsTOF Ultra 2 can be.

Oligonucleotide characterization by mass spectrometry has gained significant interest with the increased use of DNA and RNA as research reagents and pharmaceutical drug molecules. Characterization of production related impurities or degradation products is of key importance during the approval process of such biopharmaceutical products. In recent years, LC-MS based methods have become well-established tools for this characterization process. During this "Live from the Lab" session we will show from within the Bruker Demonstrations Laboratory in Bremen, how the Bruker timsTOF mass spectrometer facilitates the characterization of oligonucleotides and oligonucleotide degradation products. We will show how easy related side products can be found, characterized, and quantified automatically with the OligoQuest™ workflow within the BioPharma Compass^® Software.

We welcome you to this Live from the lab session and look forward to an interesting discussion.

Who should attend:

• Scientists interested in Oligonucleotide characterization
• Oligonucleotides QC, RNA and DNA drug development

Speakers:

• Eric Van Beelen, Ph.D., Business Development Manager Pharmaceuticals Market, Bruker Daltonics
• Detlev Suckau, Ph.D., Pharma/Biopharma Applications Software, Bruker Daltonics
• Oskar Knittelfelder, Ph.D., Field Application Scientist, Pharma/Biopharma Applications, Bruker Daltonics

In this Bruker Live from the Lab, we'll showcase the incredible potential of our cutting-edge SpatialOMx^® workflow on the timsTOF fleX. This advanced technology combines MALDI Imaging with UPLC-MS/MS PASEF^® to deliver spatially resolved molecular information and in-depth 4D-Omics from a single sample. Our team will use microGRID technology to image a mouse brain tissue slice and extract lipids for 4D-Lipidomics™, demonstrating the power of MetaboScape^® software for feature detection, lipid annotation, and statistical analysis. Don't miss this extraordinary opportunity to witness the annotated compound list visualized in SCiLS™ for distribution in the mouse brain tissue!

Single cell proteomics is growing as an analytical tool, delivering in-depth information of molecular processes occurring at the cellular level. The release of the timsTOF SCP in June 2021 opened the gate to label-free analysis of single cells. This approach removes the needs of labelling and their associated costs and bias. Removing this step is not sufficient to handle all the challenges associated with the extremely diluted sample preparation, handling and processing. In this live from the lab, we will present and demonstrate the use of a complete SCP ecosystem allowing to address the sensitivity and robustness issues at all stages of the analytical process, starting from single cell sorting and digestion down to the data analysis, considering sample transfer, separation, dia-PASEF^® acquisition and real-time data processing.

Traditional LC-based metabolomics workflows give maximum depth of information for bulk materials and are critical for untargeted profiling. Additional spatial information is possible with the versatility of the timsTOF platform and enables you to do both traditional and spatial workflows on the same instrument. MALDI Imaging is a true multiomics technique that allows for spatial determination of metabolites and lipids in tissue, as well as expanding targeted analysis to glycans and proteins. The addition of microGRID technology enables true 5 micron imaging for maximum biological information gain.

What to expect? 

• Live presentation from Bruker’s Billerica demo facility to see ease of use of the timsTOF fleX platform

Who should attend? 

• Scientists and researchers interested in expanding their metabolomics workflows to the spatial dimension, scientists working with spatial distribution of small molecule biologics
• MALDI Imaging users wanting to expand their workflows to small molecules
• Anyone interested in learning more about MALDI Imaging capabilities

Speakers 

• Kate Stumpo, Ph.D., Business Development Manager, SpatialOMx, Bruker Scientific, LLC, Billerica, MA, USA
• Josh Fischer, Ph.D., Applications Scientist, Imaging, Bruker Scientific, LLC, Billerica, MA, USA
• Ethan Yang, Ph.D., Applications Lead, Imaging, Bruker Scientific, LLC, Billerica, MA, USA

Join us on this live lab tour to learn how Sapient’s next-generation, ultra-high throughput rapid liquid chromatography-mass spectrometry (rLC-MS) platform leverages the unique bioanalytical capabilities of the Bruker timsTOF Pro 2 to enable large-scale biomarker profiling. See how the systems perform nontargeted measurement of >11,000 circulating small molecule biomarkers per biosample across thousands of samples at a time.

Data Independent Analysis (DIA) – based approaches have initially been designed to address the missing value problem in discovery proteomics experiments. Despite real benefits in terms of analysis depth and quantitative coverage, their adoption has been slowed down by the need to create a spectral library ahead of the main analysis and by the global data processing complexity. While dia-PASEF^® further increases the sensitivity, selectivity, and acquisition throughput of traditional DIA approaches, new processing pipelines, allowing to successfully perform spectral-library free analyses have emerged. Among those pipelines, which are increasing the accessibility and applicability of DIA approaches, DIA-NN seems to be one of the most promising. In this event, we are presenting TIMS DIA-NN, the first vendor-integrated version of DIA-NN, operating on Bruker’s real-tims Bruker ProteoScape™ platform. We will illustrate how real-time dia-PASEF^® data analysis with TIMS DIA-NN allows to further increase the qualitative and quantitative outcome of dia-PASEF^® experiments, as well as their throughput, and why they are to become Bruker’s preferred approach for high-performance discovery proteomics.

Join us for this virtual live demo from the Bruker lab to see the MALDI MS and MS/MS polymer analysis workflows from beginning to end. We will showcase Bruker MALDI instruments featuring advanced new technologies, show different ways to prepare samples, run polymer samples live, and demonstrate the latest software solutions for data acquisition, visualization, and interpretation. MALDI MS is the most versatile and comprehensive method of polymer analysis that can replace or augment multiple other techniques. Register for this live virtual event and see for yourself what MALDI-TOF/TOF can do for your application.

What to expect:

• Live presentation of MALDI MS instrument from Bruker's lab
• Live demo of the MALDI MS and MS/MS polymer analysis workflows: 

        -  How to operate the instrument and prepare samples in different ways
        -  How to collect the data fast and automate processing and interpretation
        -  How to spend less time on polymer analysis and get higher quality results

Who should attend:

• Material scientists and chemists
• Polymer testing, R&D and QA/QC professionals
• Lab managers

For further questions please contact polymer.support.MS@bruker.com

Protein phosphorylation is involved in many biological processes of importance and is therefore one of the most studied post-translational modifications. MS-based phosphoproteomics, however, remains challenging because of the lower ionization efficiency of phosphopeptides. In this event, we’ll illustrate how the increased sensitivity and selectivity brought by the TIMS separation, supported by Collisional Cross Section CCS-enabled bioinformatics, contributes to increase the number of detected, characterized, and quantified phosphopeptides in a 4D-Proteomics™ experiment.

Per- and polyfluoroalkyl substances (PFAS) are an important class of persistent organic pollutants and include more than 4700 compounds. Currently, environmental pollution from PFAS is very high due to decades of widespread production and use of these compounds and their chemical persistence. Comprehensive monitoring of PFAS is desirable but difficult because of the lack of reference standards and spectral libraries for compound identification.

We will present an expanded approach for comprehensive screening of PFAS based on high-resolution mass spectrometry with trapped ion mobility. In this workflow, the Kendrick mass defect of extensively fluorinated compounds is used to specifically filter for PFAS in complex matrices. PFAS identification is achieved by using a theoretical target list in combination with sophisticated tools of our MetaboScape^® software for prediction of in-silico fragments and collision cross section (CCS) values.

Curious? Then join us for the live demo!

If you are currently using immuno-imaging techniques to study protein pathways in tissue, join our virtual live demo from the lab to see how Bruker’s new MALDI HiPLEX-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue, while providing thousands of untargeted molecular images that enable deeper tissue subtyping.

This interactive session will show how easy it is to prepare samples for MALDI HiPLEX-IHC Imaging and determine the readout from acquired data. This live demo will feature the timsTOF fleX, but all Smartbeam 3D instruments are able to complete this workflow.

In the live Q&A you can ask questions directly to our experts.

Join us for this virtual live demo from the Bruker lab to see the MALDI MS polymer analysis workflows from beginning to end. We will showcase several different Bruker MALDI instruments featuring advanced new technologies, run polymer samples live, and demonstrate the latest software solutions for automated data acquisition, visualization, and interpretation. MALDI MS is the most versatile and comprehensive method of polymer analysis that can replace or augment multiple other techniques. Register for this live virtual event and see for yourself what MALDI MS can do for your application.

What to expect:

• Live presentation of MALDI MS instruments from Bruker's lab
• Live demo of the MALDI MS polymer analysis workflows:

        - How to operate the instrument and analyze different sample types
        - How to collect the data fast and automate processing and interpretation
        - How to spend less time on polymer analysis and get higher quality results

Who should attend:

• Material scientists and chemists
• Polymer testing, R&D and QA/QC professionals
• Lab managers

Oligonucleotide characterization by mass spectrometry has gained significant interest recently with the increased use of DNA and RNA as research reagents as well as pharmaceutical drug molecules. Characterization of production related impurities or degradation products is of key importance during the approval process of such biopharmaceutical products. Well established tools for this characterization process are LC-MS based methods. During this live from the lab session we will show live how Bruker’s high resolution mass spectrometers is fit for the purpose of analyzing oligonucleotides. We will demonstrate the newly developed software OligoQuest which simplifies the characterization of oligonucleotides and oligonucleotide degradation products.

Questions can be asked anytime during the session. 

We are hoping to welcome you during this live from the lab session.

Who to attend: Scientist interested in Oligonucleotide characterization, Oligonucleotides QC, RNA and DNA drug development.

If you are currently using immuno-imaging techniques to study protein pathways in tissue, join our virtual live demo from the lab to see how Bruker’s new MALDI-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue, while providing thousands of untargeted molecular images that enable deeper tissue subtyping. 

This interactive session will show how easy it is to prepare samples for MALDI-IHC Imaging and determine the readout from acquired data. This live demo will feature the timsTOF fleX, but all Smartbeam 3D instruments are able to complete this workflow.

In the live Q&A you can ask questions directly to our experts.

Access more chemical space for lead compounds and metabolites in tissue – join our virtual live lab to learn how Brukers’ timsTOF fleX MALDI-2 is a commercially unique instrument with post ionization capabilities enhancing the sensitivity of many compounds in MALDI Imaging experiments. 

This interactive session will show how timsTOF fleX and MALDI-2 can revolutionize your pharma business. 

We will demonstrate a workflow to identify and localize compound distribution information in tissue, explain how MALDI-2 can enhance the signal intensity and how TIMS increases the confidence in compound annotations. We will also highlight how our software and consumable solutions simplify MALDI Imaging.

Many of the latest vaccines and other pharmaceutical products comprise of biological molecules produced in biological systems. During the production process these biopharmaceuticals inevitably contain process-related impurities such as host cell proteins (HCPs). Since HCPs can interfere with product stability or cause safety risks to patients, removal of these impurities is an essential part of the downstream process. LC-MS based approaches are ideally suited to monitor the purification process as they provide excellent sensitivity together with protein specific identifications. 

During this Live from the lab session we will show how timsTOF Pro 2 with VIP-HESI, utilizing parallel accumulation-serial fragmentation (PASEF^®) functionality, provides ultimate sensitivity and high throughput for HCP analysis using NISTmAb reference material as sample. 

We will guide you through the workflows and explain the functionality of VIP-HESI and the timsTOF Pro 2 instrument.

We are hoping to welcome you during this live from the lab session.

Who to attend: Scientists interested in mAB analysis, Host Cell Proteins, Protein Product Purity Determination.

More details:

Sub-ppm HCP Screening in 15 minutes using dia-PASEF^®

No Compromises: Fast, Sensitive and Robust HCP Analysis with timsTOF Pro 2

Join us for this live virtual demo from Complete Phytochemical Solutions and Bruker Daltonics labs to see the MALDI-TOF MS workflows for identification of oligomeric polyphenols in dietary supplements formulated with enriched botanical extracts.  We will showcase several different Bruker MALDI instruments featuring advanced new technologies, explain the underlying principles of identity analysis and demonstrate the entire workflow from live data acquisition, to processing and interpretation.

Authentication of the botanical origin of dietary ingredients enriched in oligomeric polyphenols is challenging. MALDI-TOF MS is well suited for characterizing the unique structural features of hetero-dispersed oligomeric polyphenols found in the original botanical and enriched selective extract. MALDI-TOF MS is an advanced analytic tool that provides several significant advantages over more conventional techniques, including unparalleled throughput and speed of analysis, as well as specificity and accuracy.  Join us for this virtual event to learn more.

As a bonus, we will demonstrate a Bruker FT-NIR solution for targeted/non-targeted adulterant screening.  This tool can be used as a precursor to MALDI to quickly identify if a raw material might be contaminated.

What to expect:

• Live demonstration of MALDI MS instruments from Bruker's Lab
• Live demo of MALDI-TOF MS workflows for identification of oligomeric polyphenols in dietary ingredients from Complete Phytochemical Solutions Lab:

        -  What is the simple workflow from receipt of sample to completed analysis?
        -  How to identify unique structural features of oligomeric polyphenols with MALDI-TOF MS
        -  How to collect the data fast and automate its processing and interpretation
        -  How to spend less time on analysis and get higher quality results

Who should attend:

• Food scientists and chemists
• Food testing, R&D and QA/QC professionals
• Lab managers

The profiling of lipids from lipophilic extracts helps to get a deeper understanding in the emergence and progression of many diseases. However, this application comes with several challenges, including the presence of co-eluting isomeric or isobaric lipids. The acquisition of mobility-enhanced data using PASEF^® on timsTOF Pro 2 enhances the coverage and quality of the data – and with this the total number of compounds that can be annotated.

If you want to learn more about Brukers’ lipid profiling workflow, join us at this virtual live demo where the benefits of ion mobility separation in the 4D-Lipidomics™ workflow will be demonstrated. 

This interactive session will show live the process from the sample to result and will focus on the ease of use to set up a lipid profiling project and how software can support the analysis to give standardized high-quality reporting of the annotations.

What to expect:

• Live presentation of the timsTOF Pro 2 from the Bruker Application Lab
• Live demo of the acquisition of a complex plasma lipid extract
• Processing of data and Annotation of lipids using MetaboScape^® platform
• Time to ask questions during the live Q&A session      

Who should attend:

• Academic and pharmaceutical lipidomics and metabolomics researchers
• Lab managers

Join us for this live Proteomics demo on the timsTOF Pro 2 directly from the Bruker Application Lab.  We will demonstrate the setting up, data acquisition and analysis via nano-LC + timsTOF Pro 2 of a complex cell lysate digest sample, with Data Independent Analysis (DIA).

What to expect:

• Live presentation of the timsTOF Pro 2 from the Bruker Application Lab
• Live demo of the acquisition of a complex cell lysate including: instrument calibration, live acquisition of a complex cell lysate data set using dia-PASEF^®, review of results, discussion on software options for LFQ analysis

Who should attend:

• Academic and pharmaceutical Proteomics researchers
• Lab managers

Immunopeptides are located on major histocompatibility complex (MHC) molecules. They help the immune system to recognize foreign substances and combat infections. Due to their low abundances in general and a non-tryptic nature, high-complexity and diversity, LC-MS analysis poses special challenges.

The timsTOF SCP allows for in depth analysis of complex mixtures with ultra-high sensitivity. In addition, PASEF^® powered by trapped ion mobility (4D-Proteomics™) allows for additional separation, accumulation and accurate quantification of peptides at a high sequencing speed of up to 120 Hz. Many immunopeptides exist primarily as a singly charged species. These peptides are readily discriminated from multiply charged ion signals by their Collisional Cross Section (CCS), reducing the interference usually occurring from co-isolations in m/z only approaches. This alone is leading to cleaner MS/MS spectra.

In this webinar, we will show the benefits of the timsTOF SCP and 4D-Proteomics™ and how they improve the overall results of immunopeptidomics experiments.

Eliminate the bottleneck of data processing in your QC workflow by implementing real-time database search and smart acquisition delivered by Bruker ProteoScape™ 2022. The GPU-driven database search platform provides search results as soon as the run is done without compromising the search space and enables you to use smart acquisition to avoid the loss of precious sample. Learn more about new features in Bruker ProteoScape™ 2022 and how to integrate real-time database search in existing workflows on timsTOF Pro, fleX and SCP instruments in our upcoming webinar.

The profiling of lipids from lipophilic extracts helps to get a deeper understanding in the emergence and progression of many diseases. However, this application comes with several challenges, including the presence of co-eluting isomeric or isobaric lipids. The acquisition of mobility-enhanced data using PASEF^® on timsTOF Pro 2 enhances the coverage and quality of the data – and with this the total number of compounds that can be annotated.

If you want to learn more about Brukers’ lipid profiling workflow, join us at this virtual live demo where the benefits of ion mobility separation in the 4D-Lipidomics™ workflow will be demonstrated. This interactive session will show live the process from the sample to result and will focus on the ease of use to set up a lipid profiling project and how software can support the analysis to give standardized high-quality reporting of the annotations.

timsTOF fleX delivers molecular information in spatial context
Join us in a virtual visit to our Bruker Application Lab for a live demo of timsTOF fleX and observe how to acquire and analyze label-free molecular imaging data. Along the way, you will see some of the unique imaging features of the timsTOF fleX platform such as instant switching of the dual-source MALDI/ESI mode, Trapped Ion Mobility Separation (TIMS), and MALDI-2 enhance the depth of molecular information extracted from tissue sections and provide a spatial compliment to the intrinsic 4D-Omics capabilities of the fleX platform.

What to expect:

• Live presentation of timsTOF fleX from the Bruker Application Lab
• Live demo of measurement setup and Image acquisition including: Instrument calibration, TIMS Image acquisition, MALDI-2 enhancement of ion signal
• Live demo of SCiLS™ Lab and MetaboScape data analysis pipeline

Forensic analysis is challenged by the continuous emergence of new psychoactive substances (NPS), requiring the development of comprehensive and specific screening methods. The Toxtyper^® solution provides screening and confirmation in one analysis and can be used as a toxicology ”push button solution” with ready to use LC-MSn methods. Toxtyper^®’s screening workflows cover drugs and drugs of abuse (DOA) in a variety of samples including saliva, serum, plasma and urine.

Reliable identifications are based on a comprehensive full scan MSn library search. The manually curated Toxtyper^® spectral library contains nearly 1200 compounds. In addition, specific smaller sub libraries are available for synthetic cannabinoids and psychotropic drugs, or the large Maurer/Wissenbach/Weber library holding 6500 drugs and their metabolites for a comprehensive urine screening.

If you are interested in push-button forensic toxicology screening, join us for a live demonstration of the Toxtyper^® solution.

Understanding disease often requires comprehensive knowledge about molecular processes at their spatial occurrence. Current approaches are not seldom limited by either the analytical depth, or the lack of spatial information. We will present a unique workflow, called SpatialOMx^®, which bridges this gap by providing deep Omics data that is connected to the tissue origin through MALDI Imaging. Integration of PASEF^® empowered 4D-Lipidomics™ results from tissue extracts make lipid annotations in MALDI images more reliable through CCS-matching, which will be demonstrated using a powerful software solution.

Switch to the next level to gain higher sensitivity for a broad range of compounds. On the timsTOF fleX, the enabled MALDI-2 technology empowers your MALDI Imaging studies with unsurpassed sensitivity and ability to map more compounds. MALDI-2 is a novel two-laser technology that will significantly boost the sensitivity of many compounds by orders of magnitude and reduces the challenging ion suppression effects in MALDI. Generate more images and extract more information from your samples with MALDI-2.

TimsTOF fleX now comes with the SCiLS™ autopilot, a software workflow which allows fully automated experiment setup enabled by Bruker’s IntelliSlides^®. The SCiLS™ autopilot saves time and deskills MALDI Imaging. An important part of the new workflow also includes improvement and monitoring of the instrument performance level. Performance parameter are automatically adjusted before acquisition starts, ensuring high quality data even for beginners.

If you want to learn more about Brukers’ imaging solutions, join us at our virtual live demo where we demonstrate new powerful features on the timsTOF fleX.

Run & Done – this is the concept of our new real-time database search platform Bruker ProteoScape™. The GPU-based search algorithm allows you to access immediate database search results without compromising the search space and to use smart acquisition to avoid the loss of precious sample.

Join our virtual live demo to learn more about Bruker ProteoScape™ and how it can be seamlessly integrated in your existing proteomics workflow on the timsTOF Pro or timsTOF fleX.

Join us for this virtual live demo from the Bruker lab to see the newest MS solutions for environmental and food safety analysis in operation and witness the synergy between the targeted and untargeted analytical workflows on real life examples.  We will showcase several different Bruker QTOF and timsTOF instruments featuring advanced new technologies and demonstrate the latest software solutions for automated data acquisition, identification of known and unknown analytes, quantitation, and statistical analysis.  Register for this live virtual event and see for yourself what Bruker MS solutions can do for your application.

What to expect:

• Live presentation of QTOF and timsTOF instruments straight from the Bruker lab
• Live demo of targeted and untargeted workflows from beginning to end: What data analysis methods to use in which cases, how to use automated targeted workflows for routine analysis, how to utilize untargeted workflows to expand the capabilities of your lab, how and why to perform statistical analysis of sample batches, how to spend less time on sample analysis and get higher quality results

Join us for this virtual live demo from the Bruker lab to see the MALDI MS polymer analysis workflows from beginning to end.  We will showcase several different Bruker MALDI instruments featuring advanced new technologies, run polymer samples live, and demonstrate the latest software solutions for automated data acquisition, visualization, and interpretation.  MALDI MS is the most versatile and comprehensive method of polymer analysis that can replace or augment multiple other techniques. Register for this live virtual event and see for yourself what MALDI MS can do for your application.

What to expect:

• Live presentation of MALDI MS instruments from Bruker's lab
• Live demo of the MALDI MS polymer analysis workflows: how to operate the instrument and analyze different sample types, how to collect the data fast and automate processing and interpretation, how to spend less time on polymer analysis and get higher quality results

Join us for this virtual live Proteomics demo on the timsTOF Pro from the Bruker Application Lab.  We will demonstrate the setting up and acquisition of a complex cell lysate digest and show how Bruker ProteoScape real-time search can dramatically improve your QC workflow and reduce your data processing bottlenecks.

What to expect:

Live presentation of the timsTOF Pro from the Bruker Application Lab

Live demo of the acquisition of a complex cell lysate including: Instrument calibration, Data Acquisition with real-time “run and done” Bruker ProteoScape™ database search, Review of results, Overview of new CCS-enabled DIA and PRM workflows

Anne Kropp
Bruker Daltonics GmbH & Co. KG
Fahrenheitstrasse 4
28359 Bremen
anne.kropp@bruker.com