Protein NMR

Info: 

Introduction to protein NMR experiments on Bruker NMR spectrometers.
 

Topics:

• Concepts of protein NMR: isotope labeling and assignment strategies for protein backbone and side chains; structure calculation and protein dynamics; pulse sequence building blocks and sensitivity considerations
• Practical aspects of setup and optimization of experiments for protein backbone and sidechain resonance assignment, structure determination, and dynamics analysis
• Demonstration of the analysis of backbone resonance assignment and relaxation experiments

Remarks:

This course is designed for Bruker spectrometer users experienced with routine 1D and 2D experiments who wish to learn how to set up, run, and analyze protein NMR experiments. Concepts and particularities of protein NMR, including isotope labeling, strategies for resonance assignment, as well as structure calculation and analysis of protein dynamics will be covered in lectures. Basic pulse sequence building blocks and different types of experiments appropriate for proteins of different sizes will be discussed. In practical sessions, participants will learn how to set up and optimize 2D and 3D protein NMR experiments for resonance assignment, structure calculation, and dynamics analysis, manually in TopSpin 4 as well as using the BioTop tool. Recent developments such as non-uniform sampling and methyl-based experiments will be covered. The course also includes brief demonstrations of how to perform protein backbone resonance assignment and relaxation data analysis. Other topics of interest for the participants can be accommodated if desired.

Prerequisite Qualification:

Open to all Bruker NMR spectrometer users experienced with basic spectrometer operation and standard NMR experiments. Ideally, participants dispose of the knowledge covered in the Bruker Basic 1D and 2D NMR as well as Advanced NMR training courses.

Dates:

March 20^th - 21^st, 2025
September 25^th - 26^th, 2025

Location: