MSB | Bruker

eScience Café Seminar sponsored by BRUKER
Wednesday, July 14, 12:35 – 1:20 pm EDT

Register for our workshop at the 37th International Symposium on Microscale Separations and Bioanalysis with two talks on 4D-Proteomics™:

12:35-12:40 pm Live introduction
Gary Kruppa, Ph.D., Vice President Proteomics at Bruker Daltonics Inc., and Managing Director of Bruker S.R.O., Billerica, MA, USA

12:40 - 1:13 pm
Latest Applications of 4D-Proteomics™ Using Trapped Ion Mobility on the timsTOF Pro 2
Gary Kruppa, Ph.D., Vice President Proteomics at Bruker Daltonics Inc., and Managing Director of Bruker S.R.O., Billerica, MA, USA

4D-Proteomics – Dissecting the 3D Structure of Proteins Through Ion Mobility Enhanced Crosslinking Mass Spectrometry
Richard Scheltema, Ph.D., Assistant Professor, Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

1:13 - 1:20 pm Q&A

Speakers

Gary Kruppa, Ph.D., Vice President Proteomics at Bruker Daltonics Inc., and Managing Director of Bruker S.R.O., Billerica, MA, USA

Gary Kruppa serves as the Vice President for proteomics at Bruker Daltonics Inc. He manages a growing global team of applications, field applications and applications development scientists, focused on developing new proteomics applications for the timsTOF Pro, and supporting customers and collaborators. Dr. Kruppa earned his bachelor’s degree in chemistry at the University of Delaware and his Ph. D. in chemical physics at CalTech. Dr. Kruppa has been working with Bruker since 1991, where he first worked on the FTMS product line, later serving as Vice President for FTMS. From 2001 to 2004, Dr. Kruppa ran his own research lab at Sandia National Labs, where he and his team developed novel methods and reagents for chemical crosslinking of proteins for the study of protein structure. In 2004 he rejoined Bruker, where he served as Vice President for Business Development until 2014. From 2014 to 2016 Dr. Kruppa worked as CEO of MRM Proteomics Inc., to commercialize kits for quantitative MRM proteomics analysis of potential biomarkers. Dr. Kruppa returned to Bruker in 2016 to serve as Vice President for proteomics, where he helped launch the revolutionary timsTOF Pro QTOF Mass Spectrometer powered by the PASEF^® method.

Richard Scheltema, Ph.D., Assistant Professor, Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Richard currently runs his own, independent research group at Utrecht University. After finalizing his PostDoc working on LC-MS/MS platforms within the group of Prof. Dr. Matthias Mann (Max Planck Institute of Biochemistry, DE), he started the Scheltema laboratory at Utrecht University. The focus lies on structural proteomics applied to medium & high complexity protein environments like the ECM and membrane proteins. Mass spectrometry based approaches are employed that are independent of protein size, deal with high complexity mixtures and play well with other structural biology techniques like Cryo-EM and crystallography to uncover novel and exciting biology. The technological developments and their applications have been published in journals like Nature Protocols, Nature Communications, Molecular Cell, Cell Reports, ACS Central Science, and PNAS and additionally have been commercialized and are worldwide in use.

Literature and Posters

Videos

Evosep Whisper

Webinar: Whisper is coming

Learn more about the advantages of the pioneering Whisper™ flow technology in our webinar hosted by Nicolai Bach, Head of Applications at Evosep, from December 15th.

view on demand

Evosep Whisper - Literature

Evotips

High sensitivity Evotips for analyzing low sample amounts

For more efficient peptide recovery of small sample loads (< ~100 ng), Evosep recommends High Sensitivity Evotips, that have superior performance at very low loads and almost identical retention times for other applications.

High Sensitivity Evotips provide significantly more IDs for low sample amounts, with both our standard methods as well as with our Whisper methods specifically designed for ultra sensitive applications.

Brochure: High Sensitivity Evotips

For Research Use Only. Not for use in clinical diagnostic procedures.

37th International Symposium on Microscale Separations and Bioanalysis

eScience Café Seminar sponsored by BRUKER Wednesday, July 14, 12:35 – 1:20 pm EDT