Protein Analyzer

CONFOCHECK

The CONFOCHECK is a dedicated FT-IR system for the investigation of proteins in water. Its specific configuration facilitates a fast data acquisition (ca. 30 sec. per sample) with a high sample throughput controlled by an extremely user-friendly software interface.

Answers about Protein Dynamics.

And Other Structural Parameters.

Secondary Structure Determination in Proteins using FT-IR

Main applications for the CONFOCHECK are the detection and quantification of conformational changes in proteins and the protein concentration determination. Quantitative determination of the protein concentration and the secondary structure elements (alpha-helix, beta-sheet) from the analyzed protein samples is accomplished within seconds via internal calibrations.

The CONFOCHECK is equipped with several specific protein sampling cuvettes that allow the measurement of solved and membrane bound proteins, even at very low amounts (10 µg). To reach optimal temperature constancy and to facilitate the measurement of temperature induced protein conformational changes, the sample cuvettes are temperature-controlled by a thermostat.

Watch how we monitor temperature induced conformational changes and quantify proteins.

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In this video we show how to use CONFOCHECK for protein stability characterization.

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What is the confocheck

The Complete Protein Analysis Solution

FT-IR Protein Applications:

Protein quantification
Detection of conformational changes
Protein dynamics (temperature induced conformational changes)
Monitoring of conformational changes during protein aggregation, precipitation and crystallization
Determination of the secondary structure
Quantification of all kind of solutes in aqueous samples (buffers, excipients, detergents)

Analyzing Proteins in Pharma:

Pre-formulation and formulation development: Analysis of the influence of the formulation on protein stability
Stability studies (stress tests): Influence of pH, temperature, mutation, etc. on the protein stability
Quality control: Check of protein concentration, structure on correct product composition (buffer, excipients) in production environment
Binding studies: Analysis of the impact of ligand-binding on the protein structure

CONFOCHECK Benefits:

Complete system can be validated to use in pharmaceutical quality control.
Optimized, long-life components to minimize maintenance and prolong revalidation cycles.
Gold optics for maximum sensitivity in the MIR
10 year warranty on laser
10 year warranty on interferometer
5 year warranty on MIR source

Study proteins in water

AquaSpec Transmission Cell

The AquaSpec™ is used for the measurement of water soluble proteins or solubilized membrane-bound proteins. Using the internal calibrations of the CONFOCHECK the secondary structure and concentration of the analyzed protein are determined directly after measurement (ca. 30 sec).

It features a flow-through transmission cell, with an optimal path length of ~7 µm. The AquaSpec™ also permits higher sample throughput and accuracy. Easily measure low protein concentrations (below 0.1 mg/ml) and detect the smallest conformational changes.

Using the AquaSpec^TM transmission cell the highest sensitivity for the detection of conformational changes and the best accuracy in the protein concentration determination is achieved.

Applications

Determination of changes in protein structure
Protein concentration determination
Quality control of biopharmaceuticals
Secondary structure determination
Quantification of various substances in aqueous solutions

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Monitor conformational changes

BioATR II

The BioATR II is a micro-ATR unit that was specially developed for the investigation of temperature-induced unfolding, refolding and denaturation processes of proteins in aqueous media.

It can be applied for water solved and also for membrane-standing proteins. The number of internal reflections in the ATR crystal (silicon) is adjusted to result in an optimal protein signal in aqueous samples.

Applications:

Automated temperature ramps (0-95°C):
Analysis of temperature-induced conformational changes
Measurement of phase transitions in lipids
Quality control of biopharmaceuticals
Membrane-standing proteins
Conformational changes induced by ligand-binding, pH-changes, salts

The BioATR II is temperature-controlled by the same thermostat like the other accessories that are part of the CONFOCHECK system. With this unit temperature ramps from 0 to 95°C can be performed automatically to analyze the temperature-induced structural changes and the protein melting point.

Furthermore, you can characterize fibrillation and aggregation processes and monitor protein structural changes in dependence of pH‑changes or ligand-binding interactions. Using a special dialysis top section for BioATR II, low molecular weight substances can be dialyzed into the protein compartment without diluting the protein.