Destriping Module

Light-sheet microscopes, with their orthogonal illumination-detection geometry,* are especially prone to induce striping artifacts in images.

When imaging biological samples with light-sheet microscopy, inhomogeneities in the sample (e.g., pigments or cell nuclei) can absorb or scatter light from the excitation beam. This casts shadows behind the obstacle and causes striping artifacts.

Our destriping module mitigates the effect of such obstacles, minimizing shadows and striping as it:

• Illuminates the sample from different angles during image acquisition; and
• Enables the excitation light to reach regions behind obstacles.

Moreover, the destriping module’s beam rotation is much faster than the SPIM camera exposure time, allowing users to generate a homogenous illumination profile and thus minimize artifacts without compromising acquisition speed.

* Optical arrangement with the illumination source located orthogonally (perpendicular) relative to the detection axis.

▲ Cleared mouse embryo. Left: Image collected without the destriping module showing striping artifacts. Right: Image collected with the destriping module showing successful removal of striping artifacts.