SKYSCAN 1272
Highlights
SKYSCAN 1272 – High-resolution XRM
The desktop SKYSCAN 1272 is an innovative high-resolution 3D X-ray microscope based on micro computed tomography (micro-CT) technology, which can non-destructively visualize with more than 200 Megapixel (14450 x 14450 pixels) in every virtual slice through an object.
The ability to move both the sample and large-format CCD camera as close as possible to the source substantially increases the measured intensity. That is why SKYSCAN 1272 scans up to 5 times faster compared to conventional systems with fixed camera position.
The SKYSCAN 1272 offers automatic selection of parameters with Genius-Mode. Magnification, energy, filter, exposure time and background corrections can all be optimized automatically with a single click.
A 16-position sample changer is optionally available for unattended high throughput scanning.
SKYSCAN 1272 is complemented by 3D.SUITE. This comprehensive software suite covers GPU-accelerated reconstruction, 2D/ 3D morphological analysis, as well as surface and volume rendering visualization.
Key Features
Genius Mode
The SKYSCAN 1272 offers automatic selection of parameters with Genius-Mode. Magnification, energy, filter, exposure time and background corrections can all be optimized automatically with a single click.
Furthermore, both the sample and large-format CCD camera can be positioned as close as possible to the source, which substantially increases the measured intensity. That is why SKYSCAN 1272 scans up to 5 times faster compared to conventional systems with fixed camera position.
Sample changer
SKYSCAN 1272 can optionally be equipped with an external 16-position sample changer to increase throughput for QC and routine analysis.
The sample changer accepts a variety of sample sizes, up to a diameter of 25 mm.
Samples can be can be easily replaced at any time without interrupting an ongoing scanning process. New samples are automatically detected, and LED‘s indicate the status for every scan: ready, scanning, done.
In-situ stages
The Bruker material testing stages are designed to perform compression experiments up to 4400 N and tensile experiments up to 440 N. All stages automatically communicate through the system’s rotation stage, without the need of any cable connections. Using the supplied software, scheduled scanning experiments can be set up.
Bruker's heating and cooling stages can reach temperatures of up to +80ºC, or 30ºC below ambient temperature. Just like the other stages, no extra connections are needed, and there is an automatic recognition of the stage. Using the heating & cooling stages, samples can be examined under non-ambient conditions, to evaluate the effect of temperature on the sample’s microstructure.
Highlighted Applications
Bone Applications
The SKYSCAN 1272 succeeds the SKYSCAN 1072 and SKYSCAN 1172 models, between them accounting for at least half of the entire microCT bone morphometry literature. It’s the benchmark for high resolution with high throughput and ease of use. This makes it the ideal solution for bone disease models from osteoporosis and arthritis to bone tumor and myeloma, and for large scale gene phenotyping.
- Ultimate performance in a desktop model, voxel size to 0.4um, x-ray voltage 20-100kV, provide resolution and contrast to analyze bone osteocyte lacunae and vessel canals.
- High throughput and automated batch scanning including an optional sample changer for 16 preloaded samples, a powerful set-up for genetic screening of thousands of phenotypes, of mice or zebrafish for trabecular and cortical bone parameters.
- Advanced 3D image analysis functions include phase contrast / retrieval (Paganin, small angle), 3D registration, allowing flexible solutions for all disease models and analysis requirements.
- Bone morphometry (ASBMR nomenclature) with comprehensive 3D and 2D parameters, with densitometry including BMD calibration references over the preclinical size range.
Soft tissues
Ex vivo scanning of biological tissues is a very good way to show their internal structures non-destructively. A contrast agent or chemical drying can improve the image quality by further enhancing or differentiating densities. The SKYSCAN 1272 is the ideal platform for such imaging with its resolution, easy sample handling and high throughput – that’s why you will find so many publications using this scanner.
- Typical applications for this system are the visualization of tumor growth or vascularization in the mouse lung. Emphysema and fibrosis are also clearly visualized.
- Ultimate performance in a desktop model, voxel size to 0.4um, x-ray voltage 20-100kV, provide resolution and contrast down to the lung alveolar level.
- Stabilizing these fragile or wet tissues during scanning can be challenging, a special set of Bruker developed specimen chambers can take care of this.
- Full in-house software solution for lung tumor morphometric analysis, auto-separation of lung from other tissues in CT-Analyser software.
Plant and Animal Biology
MicroCT is exceptionally good for the visualization of internal structures in the finest details of the tissues of plants and animals. This imaging method differentiates between densities without harming or destroying the scanned object. That’s why zoology and botany are fast-growing microCT applications with SKYSCAN 1272 users at the forefront. A wide range of different living organisms can be visualized and analyzed with minimal or no sample-treatment.
- High resolution scanning with object detection down to 0.4um combined with an 11 or 16 megapixel camera for high resolution in a useful volume.
- Due to the continuous voltage range of the sources, and 6 internal energy filters, the perfect scan set up for each different sample can be guaranteed.
- Comprehensive 3D image analysis capability including morphometry and densitometry, phase contrast / retrieval (Paganin, small angle), 3D registration, segmentation and advanced image processing methods.
Application Gallery
Cross-section through a kidney stone scanned at 2µm voxel size, showing different phases. (Sample provided by the Laboratory of Clinical Chemistry, University Hospital of Liège, Sart-Tilman, Belgium.
3D model of a mouse femur scanned at 5 µm voxel size, color-coded for local thickness.
Cross-section through a mouse tibia, scanned at 800 nm voxel size, showing the osteocyte lacunae.
3D rendered image of a mouse tibia, scanned at 800 nm voxel size, showing the osteocyte (blue) and vascular (red) network.
Cross-section through a mouse lung, scanned at 1 micron voxel size.
Stag beetle scanned at 10 µm voxel size. Sample provided by the University of Antwerp, Belgium.
Coffee bean scanned at 5 µm voxel size.
3D rendering of the internal structure of a rose flower.
3D rendering of a PLA scaffold pore network color-coded for local thickness, scanned at 1.5 µm voxel size.
Cross-sections through a wood sample scanned 875 nm voxel size.
3D render of a sand bubbler crab scanned at 8.9 µm voxel size.
Shell of a sea urchin scanned at 17µm pixel size.
Orthogonal slices through a mouse embryo (E16.5), scanned at 1.75 µm voxel size.
Volume rendered 3D model of a mouse lung vasculature and airways, scanned after chemical drying at 10 µm voxel size. A necrotic area can be observed in the centre of the left lung.
3D models of a bee head; scanned at high resolution after PTA staining. The right model is virtually cut to expose the internal structures. (sample from the University of Granada, Spain)
SKYSCAN 1272 CMOS Specifications
Feature |
Specification |
Benefit |
X-ray source |
40 – 100 kV 10 W < 5 µm spot size at 4 W |
Maintenance-free sealed X-ray source Fast scans for QC, or 4D XRM |
X-ray detector |
16 MP sCMOS detector (4096 x 4096 pixels) | Fine-pitched detectors for achieving highest resolution |
Object size |
75 mm diameter 80 mm height |
Capable to scan from small to medium large sample sizes |
Sample changer (optional) |
16 samples up to 25 mm diameter External access |
Unattended high throughput Any combination of large and small samples Add/remove samples at any time without interrupting the actual scan |
Dimensions |
W 1160 mm x D 520 mm x H 330 mm Weight 150 kg |
Space-saving desktop system that fits in every lab |
Power supply |
100-240V AC, 50-60Hz, 3A max. |
Minimum installation requirements, a standard power supply suffices |
POSITION, SCAN, RECONSTRUCT and ANALYZE
Bruker XRM solutions include all software needed to collect and analyze data. An intuitive graphical user interface with user guided parameter optimization support both expert and novice users. By using the latest GPU powered algorithms, reconstruction time is substantially reduced. CTVOX, CTAN and CTVOL combine to form a powerful suite of software for both qualitative and quantitative analysis of models.
Measurement Software:
SKYSCAN 1272 – Instrument control, measurement planning and collection
Reconstruction Software:
NRECON – Transforms the 2D projection images into 3D volumes
Analysis Software:
DATAVIEWER – Slice-by-slice inspection of 3D volumes and 2D/3D image registration
CTVOX – Realistic visualization by volume rendering
CTAN – 2D/3D image analysis & processing
CTVOL – Visualization of surface models to export for CAD or 3D printing
News & Events
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What is XRM?
SKYSCAN 1272 - Service & Support
Available services include
- Help desk support from highly-skilled troubleshooting professionals, to isolate and resolve hard- and software problems
- Web-based remote instrument service for service diagnosis and applications support
- Merged reality support with Help Lightning – a virtual engineer at your side (video)
- Planned maintenance, according to your requirement
- Customer on-site repair and maintenance service
- Spare parts availability typically over night or within a few working days worldwide
- Compliance services for installation qualification, operational qualification / performance verification
- Site planning and relocation
Bruker Support
On our support website you will find:
Software Updates
- System software updates are available online to registered users.
Documentation
- Product manuals and installation guides are available online to registered users.
LabScape
Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging
Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.
LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory
