Light-Sheet Microscopy Tutorial Videos

MuVi SPIM- Octagon Exchange from Cleared Sample to Live Sample Imaging

The video shows the fast and easy transition from Cleared Sample (CS) to Live Sample (LS) imaging with the light-sheet microscope MuVi SPIM from Luxendo.

The transition is easily achieved by exchanging the Octagon, which is the core unit carrying illumination and detection objective lenses, and the sample chamber.

MuVi SPIM - Octagon Exchange from Live Sample to Cleared Sample Imaging

The video shows the fast and easy transition from Live sample (LS) to Cleared Sample imaging with the light-sheet microscope MuVi SPIM from Luxendo.

The transition is easily achieved by exchanging the Octagon, which is the core unit carrying illumination and detection objective lenses and the sample chamber.

MuVi SPIM LS - Sample Mounting

The video shows how to mount a sample (e.g. Zebrafish embryo) in the MuVi SPIM LS from Luxendo.

The steps include preparing the capillaries that will hold your sample, fishing the sample, and inserting the capillary in the sample holder.

The MuVi SPIM features a horizontal setup and is designed for fast imaging of large samples, both living and cleared tissue. The unique 4-axis concept with its two-sided illumination facilitates four orthogonal views of the sample without the need for rotation.

MuVi SPIM CS - Cleared Sample Octagon - Exchange 10x to 20x Objective Lens

The video shows in a few simple steps how to exchange the 20x to the 10x detection lens in the cleared sample octagon.

The Luxendo’s MuVi SPIM CS is an extension of the MuVi SPIM capabilities for 3D imaging of optically cleared samples. The system features an exchangeable cleared sample octagon, which has been cleverly designed to enable flexible configurations of detection lenses (10x and 20x).

MuVi SPIM CS Maintenance - Cleaning the Octagon

The video shows how to maintain the cleared sample Octagon. It is recommended to clean the sample chamber before using it, especially in a multi-user environment. Cleaning the chamber is mandatory when changing clearing solutions.

The Luxendo’s MuVi SPIM CS is an extension of the MuVi SPIM capabilities for 3D imaging of optically cleared samples.

MuVi SPIM CS - Measuring the Refractive Index

The video shows how to measure the refractive index of the clearing solution used in your experiment. This step is necessary to adjust the correction collar of the detection objectives lenses in the cleared sample (CS) Octagon.

The Luxendo’s MuVi SPIM CS is an extension of the MuVi SPIM capabilities for 3D imaging of optically cleared samples.