InsightCell for High-Field

InsightCell™ is based on the established InsightMR™ platform, bringing Bruker’s trusted expertise in flow NMR to the study of living cells. It is designed for real-time on-line metabolite monitoring and enables accurate quantification of dynamic metabolic changes. InsightCell offers a powerful window into cellular function while keeping your bioprocess running continuously.

• Continuous Bioprocess Monitoring: Whether feeding cells with fresh media inside the flow tube or transferring culture fluids directly from bioreactors, InsightCell tracks metabolic changes as they happen.
• Fermentation Optimization: Monitor key metabolites like ethanol or lactate in real time. Use this data to fine-tune pH and temperature, improving efficiency and reproducibility.
• Versatility Across Applications: From antibiotics and vaccine production to vitamin synthesis and enzyme engineering, InsightCell empowers you to control and optimize critical bioprocess parameters.

InsightCell

• Biocompatible flow cell for live-cell cultures
• Low-shear design preserves cell viability
• Temperature-controlled transfer lines: -20 to 120 °C capability
• Compatible with standard high-field NMR probes
• Compatible with all Bruker sample changers
• InsightMR 2.0 software  for automation and analysis

InsightMR Software 2.0

InsightMR is a software platform for real-time analysis of chemical and bioprocesses using on-line and in-line NMR. It allows on-the-fly adjustment of acquisition parameters, supports a wide range of experiments, and handles large series of spectra efficiently. Data are seamlessly transferred to Mnova for further analysis, with results delivered as ready-to-use kinetic and waterfall plots.

• Real-time metabolite profiling without sampling interruptions
• Continuous media exchange during NMR acquisition
• Direct connection to bioreactors and perfusion systems
• Straightforward installation and operation with minimal learning curve

Online monitoring of living cells metabolism by nuclear magnetic resonance:

https://www.bioaster.org/fr/un-projet-avec-bioaster/study-cases/insightcell/